LPS刺激肥大细胞炎症因子分泌过程中miRNA的表达差异
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国家自然科学基金面上项目 ( 81370132);南京市医学科技发展项目 (201108012)


miRNA expression differences in inflammatory cytokines secretion in LPS-stimulated mast cells
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    摘要:

    目的:研究在细菌脂多糖(lipopolysaccharide,LPS)刺激肥大细胞炎症因子分泌过程中miRNA的表达差异-方法: 肥大细胞P815培养后,用LPS(1 μg/ml)激发肥大细胞,16 h后终止反应,通过酶联免疫吸附实验(ELISA)检测上清中肿瘤坏死因子α(TNF-α)-白介素-6(IL-6)的水平-再收集细胞,通过实时荧光定量PCR法检测miRNA的改变-结果:在LPS刺激肥大细胞后,TNF-α- IL-6的分泌与基础分泌量相比明显升高,差异有统计学意义-同时在肥大细胞中干预组miRNA-126的表达是正常组的1.3倍(P < 0.05),干预组miRNA-155的表达是正常组的1.2倍(P < 0.05),干预组miRNA-223的表达是正常组的1.7倍(P < 0.05),干预组miRNA-221的表达是正常组的1.2倍(P < 0.05),干预组miRNA-192的表达是正常组的1.4倍(P < 0.05)-结论: miRNA在炎症因子LPS刺激肥大细胞P815后存在差异性表达,而这些差异表达的miRNA可能与肥大细胞的功能调节存在相关性-

    Abstract:

    Objective:To study the difference of expression of miRNAs of inflammatory cytokines in lipoplysaccharide(LPS)-stimulated mast cell secretion. Methods:P815 cells were stimulated by LPS (1 μg/ml) for 16 hours. The expression levels of TNF-α and IL-6 in supernatant were detected by ELISA. Real-time PCR was performed to detect the expression differcence of miRNAs after the cells were recollected. Results:In P815 after LPS stimulation,the secretions of TNF-α and IL-6 were largely up-regulated than those of control (P < 0.05). Five miRNAs in the intervention group were found increasingly expressed compared with those in the control group,with miRNA-126 1.3-fold,miRNA-155 1.2-fold,miRNA-223 1.7-fold,miRNA-221 1.2-fold,and miRNA-192 1.4-fold (P < 0.05). Conclusion: miRNAs were expressed differentially in mast cells after LPS stimulation. The differcent expressions of miRNA may be invovled in mast cells function changes.

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莫珍珍,周 瑶,徐 红,刘 峰,赵德育. LPS刺激肥大细胞炎症因子分泌过程中miRNA的表达差异[J].南京医科大学学报(自然科学版),2014,(10):1317-1319-1341

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  • 收稿日期:2014-05-03
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  • 在线发布日期: 2014-11-02
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