LPS刺激肥大细胞炎症因子分泌过程中miRNA的表达差异
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金面上项目 ( 81370132);南京市医学科技发展项目 (201108012)


miRNA expression differences in inflammatory cytokines secretion in LPS-stimulated mast cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究在细菌脂多糖(lipopolysaccharide,LPS)刺激肥大细胞炎症因子分泌过程中miRNA的表达差异-方法: 肥大细胞P815培养后,用LPS(1 μg/ml)激发肥大细胞,16 h后终止反应,通过酶联免疫吸附实验(ELISA)检测上清中肿瘤坏死因子α(TNF-α)-白介素-6(IL-6)的水平-再收集细胞,通过实时荧光定量PCR法检测miRNA的改变-结果:在LPS刺激肥大细胞后,TNF-α- IL-6的分泌与基础分泌量相比明显升高,差异有统计学意义-同时在肥大细胞中干预组miRNA-126的表达是正常组的1.3倍(P < 0.05),干预组miRNA-155的表达是正常组的1.2倍(P < 0.05),干预组miRNA-223的表达是正常组的1.7倍(P < 0.05),干预组miRNA-221的表达是正常组的1.2倍(P < 0.05),干预组miRNA-192的表达是正常组的1.4倍(P < 0.05)-结论: miRNA在炎症因子LPS刺激肥大细胞P815后存在差异性表达,而这些差异表达的miRNA可能与肥大细胞的功能调节存在相关性-

    Abstract:

    Objective:To study the difference of expression of miRNAs of inflammatory cytokines in lipoplysaccharide(LPS)-stimulated mast cell secretion. Methods:P815 cells were stimulated by LPS (1 μg/ml) for 16 hours. The expression levels of TNF-α and IL-6 in supernatant were detected by ELISA. Real-time PCR was performed to detect the expression differcence of miRNAs after the cells were recollected. Results:In P815 after LPS stimulation,the secretions of TNF-α and IL-6 were largely up-regulated than those of control (P < 0.05). Five miRNAs in the intervention group were found increasingly expressed compared with those in the control group,with miRNA-126 1.3-fold,miRNA-155 1.2-fold,miRNA-223 1.7-fold,miRNA-221 1.2-fold,and miRNA-192 1.4-fold (P < 0.05). Conclusion: miRNAs were expressed differentially in mast cells after LPS stimulation. The differcent expressions of miRNA may be invovled in mast cells function changes.

    参考文献
    相似文献
    引证文献
引用本文

莫珍珍,周 瑶,徐 红,刘 峰,赵德育. LPS刺激肥大细胞炎症因子分泌过程中miRNA的表达差异[J].南京医科大学学报(自然科学版),2014,(10):1317-1319-1341

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2014-05-03
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2014-11-02
  • 出版日期: