维生素A对SD大鼠脑组织MC4R、POMC基因表达的影响
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Effect of vitamin A on appetite and gene expression of MC4R and POMC in rats
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    摘要:

    目的:探讨维生素A对SD大鼠食欲和黑皮素4受体(MC4R)-阿片黑素促皮质激素原(POMC)基因表达的影响,初步探讨维生素A影响大鼠食欲的机制-方法:将雄性SD大鼠随机分为A组(维生素A缺乏组)和B组(对照组)-喂养74 d,实验的第75天A组随机取出16只,分为A1组(维生素A缺乏组)和A2组(维生素A皮下补充组);B组随机取出8只,为B1组(对照组)-实验第79天,将这3组大鼠全部处死,取血和组织进行相关指标测定-A组剩余的动物按体重随机分为A3组(维生素A缺乏组)和A4组(维生素A食物补充组)-B组剩余的8只动物为B2组(对照组)-30 d后将其全部处死,取组织进行相关指标测定-结果:维生素A缺乏组进食量显著低于正常对照组,给予缺乏组大鼠维生素A皮下和食物补充后,大鼠进食量显著增多-维生素A缺乏组MC4R和POMC的mRNA表达升高,补充维生素 A后大鼠POMC mRNA的表达水平明显降低-结论:维生素A影响大鼠食欲的可能机制是改变了下丘脑有关控制食欲的基因表达-

    Abstract:

    Objective:To investigate the effects of vitamin A on appetite and the gene expression of MC4R, POMC, and to explore the mechanism of vitamin A on rats’ appetite. Methods: Male Sprgue-Dawley (SD) rats were divided into two groups: group A (the vitamin A deficient group) and group B (the vitamin A normal control group). Seventy-four days later, 16 rats were selected from group A and then divided into two groups: group A1 (the vitamin A deficient group) and group A2 (the vitamin A administration group). Also 8 rats were selected from group B as group B1 (the vitamin A normal control group). At the 79th day, all the rats in the three groups were sacrificed, and some relevant indices were measured. The remaining rats in group A were divided into two groups again: group A3 (the vitamin A deficient group) and group A4 (the vitamin A supplement group). The remaining 8 rats in group B was group B2 (the vitamin A normal control group). All the rats were sacrificed after 30 days. Some relevant indices were also measured. Results: In the VAD group, the total food intake was significantly lower than that of the control group. After giving vitamin A supplement, the total food intake obviously increased. The expression of MC4R and POMC in the VAD group was increased, while POMC was decreased obviously after vitamin A supplement. Conclusion: Vitamin A affects the expression of related appetite regulator, resulting in the change of food in take.

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李 芹,徐济达,王 嵘,敖淑清,张 娟,陆晓梅.维生素A对SD大鼠脑组织MC4R、POMC基因表达的影响[J].南京医科大学学报(自然科学版),2014,(10):1342-1345

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  • 收稿日期:2013-11-14
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  • 在线发布日期: 2014-11-02
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