文章摘要
陈文标,黄建溶,喻祥琪,彭武建,林小聪,戴 勇.高通量测序筛查特发性膜性肾病患者外周血细胞microRNA的差异性表达[J].南京医科大学学报,2014,(11):1507,1514~1541
高通量测序筛查特发性膜性肾病患者外周血细胞microRNA的差异性表达
Identification of different expression of microRNA in membranous nephropathy using high-throughput sequencing technology
投稿时间:2014-02-19  
DOI:10.7655/NYDXBNS20141110
中文关键词: 膜性肾病  小核糖核酸  高通量测序  差异性表达  碱基编辑
英文关键词: membranceous nephrology  microRNA  high-throughput sequencing  diffenent expression  base edit
基金项目:深圳市卫生局科技项目(201202139)
作者单位
陈文标 暨南大学第二临床学院,深圳市人民医院临床医学研究中心, 广东 深圳 518020 
黄建溶 深圳市第三人民医院肾脏内科,广东 深圳 518112 
喻祥琪 暨南大学第二临床学院,深圳市人民医院临床医学研究中心, 广东 深圳 518020 
彭武建 深圳市第三人民医院肾脏内科,广东 深圳 518112 
林小聪 广东医学院生物化学研究所,广东 湛江 524023 
戴 勇 暨南大学第二临床学院,深圳市人民医院临床医学研究中心, 广东 深圳 518020 
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中文摘要:
      目的:寻找特发性膜性肾病(idiopathic membranous nephropathy,IMN)患者外周血单个核细胞小核糖核苷酸(microRNA)的差异性表达以及发生碱基突变的难易度?方法:运用高通量测序技术分别对IMN患者和健康对照者(NC)的microRNA进行测序,构建IMN与NC组之间microRNA差异性表达谱?并对两组的microRNA进行碱基编辑分析,寻找发生了碱基突变的microRNA,比较IMN与NC组 microRNA碱基突变的难易程度?结果:通过构建表达谱,找到了has-miR-208b?has-miR-195-3p?has-miR-23b-5p?has-miR-95?has-miR-503?has-miR-449a?has-miR-486-3p与has-miR-27 8个microRNAs最具有差异性表达?2组共同表达的41个microRNA中,IMN比NC更容易发生碱基编辑而引起碱基突变?结论:IMN患者与健康对照者的microRNA存在着差异性表达,差异性表达的microRNA特异性很高,可以作为深入研究IMN发病机制的靶点?IMN患者相比健康对照者更容易发生microRNA碱基编辑引起碱基突变,这些发生碱基突变的microRNA可能用于解释IMN的病因基础?
英文摘要:
      Objective:To identify microRNA with different expression and the ease on base mutation based on peripheral blood mononuclear cell from idiopathic membranous nephropathy (IMN) patients. Methods:We used the high-throughput sequencing technology to establish the differently expressed spectrum of microRNA of IMN patients and healthy people (the control group),respectively. We found out the significantly different expression of microRNA. We also analyzed the base edited in the microRNA to find out the microRNA which happened in base mutation, to compare the microRNA between the two groups which in common to evaluate the ease of base mutation. Results:After the creation of microRNA different expression profile,we found out eight microRNAs,which were most significantly different expression,including has-miR-27,has-miR-208b,has-miR-195-3p,has-miR-23b-5p,has-miR-95,has-miR-503,has-miR-449a and has-miR-486-3p. In the common microRNAs between the IMN and the NC groups,there are forty one microRNAs,which had the greater opportunity to have the base edited in the IMN group than in the NC groups. Conclusion:The microRNA expression between the IMN and the NC groups showed significant differences,and the significantly different expression microRNAs were special that could be used as target to further research the pathogenesis of IMN. The microRNAs of IMN patients were more easily to have base edited than that of NC group,and the microRNAs with edited base coulbe possibly related to the pathological mechanism of IMN.
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