文章摘要
席 勇,王树金,李云龙,夏 炎,任永信.AGEs诱导软骨细胞凋亡和影响细胞外基质变化的研究[J].南京医科大学学报,2014,(12):1649~1653
AGEs诱导软骨细胞凋亡和影响细胞外基质变化的研究
Advanced Glycation End Products induced apoptosis and expression of extracellular matrix in mouse chondrocytes
投稿时间:2014-06-19  
DOI:10.7655/NYDXBNS20141205
中文关键词: 骨性关节炎  AGEs  细胞凋亡  细胞外基质
英文关键词: Osteoarthritis  AGEs  apoptosis  extracellular matrix
基金项目:国家重点基础研究发展计划(973计划,2014CB942903)
作者单位
席 勇 南京医科大学第一附属医院骨科,江苏 南京 210029
丹阳市人民医院骨科,江苏 丹阳 212300 
王树金 丹阳市人民医院骨科,江苏 丹阳 212300 
李云龙 丹阳市人民医院骨科,江苏 丹阳 212301 
夏 炎 丹阳市人民医院骨科,江苏 丹阳 212302 
任永信 南京医科大学第一附属医院骨科,江苏 南京 210029 
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中文摘要:
      目的:明确晚期糖基化终末产物(advanced glycation end products,AGEs)诱导小鼠关节软骨细胞凋亡情况和影响细胞外基质变化?方法:体外分离培养小鼠软骨关节细胞,给予不同浓度AGEs共培养后,分别于24 h和48 h时间点观察细胞凋亡情况;通过Western blot方法检测Ⅰ型胶原?MMP-3?MMP-9以及p53蛋白的表达情况?结果:不同浓度AGEs处理软骨细胞24 h后,与对照组比较,细胞凋亡率出现升高(P < 0.05,n=3);与BSA组比较,200 mg/L浓度的AGEs组细胞凋亡率明显升高(P < 0.05,n=3),并且随着AGEs浓度和培养时间增加,软骨细胞的凋亡率也增加?200 mg/L的浓度AGEs处理软骨细胞48 h后,Ⅰ型胶原蛋白的表达显著下降(P < 0.05,n=3);另外,50?100?200 mg/L的浓度AGEs处理软骨细胞48 h后,p53?MMP-3?MMP-9的表达显著高于对照组和BSA组(P < 0.05,n=3)?结论:AGEs能诱导小鼠软骨细胞凋亡,并刺激软骨细胞p53?MMP-3?MMP-9表达增多,降低Ⅰ型胶原蛋白表达?
英文摘要:
      Objective:To investigate the effects of advanced glycation end products (AGEs) on apoptosis and approach the mechanism. Methods:The primary cultured mouse chondrocytes were incubated with different concentration of AGEs for 24 h or 48 h. Apoptosis of chondrocytes was detected by FCW;expression of ColⅠ,MMP-3,MMP-9 and p53 was measured by Western blot. Results:After the chondrocytes were treatment with AGEs(50,100,200 mg/L) for 24 h,the apoptosis was increased compared with the control groups;the maximum stimulation of chondrocytes was 200 mg/L AGEs. After the chondrocytes were treatment with AGEs(50,100,200 mg/L) for 48 h,expression of Col was significamtly decreased(P<0.05,n=3) while the levels of MMP-3,MMP-9 and p53 were increased,compared to control groups and BSA group. Conclusion:AGEs can increase the apoptosis of mouse chondrocytes in a dose-dependent manner, thus damage in chondrocytes via decrease of the expression of extracellular matrix.
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