PLGA经多巴胺改性后负载纤连蛋白对MC3T3-E1细胞黏附的影响
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江苏省自然科学基金(BK20140911);江苏高校优势学科建设工程资助(2011-137);江苏省医学领军人才与创新团队


Effects of fibronectin modified PLGA membranes via dopamine on cell adhesion of MC3T3-E1 cells
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    摘要:

    目的:建立以聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]为基底,经多巴胺(dopamine,DP)负载纤连蛋白(fibronectin,FN)的材料模型,研究FN对MC3T3-E1细胞黏附的影响。方法:使用红外光谱分析仪,接触角仪和石英晶体损耗检验微量天平测量样品表面基团-膜的亲水性和FN负载量;在磷酸盐缓冲液(PBS)环境中,分别接种MC3T3-E1细胞于各样品,使用延时照相系统每隔3 min观察细胞黏附情况,CCK-8法测量接种细胞后0.5-1.0-4.0-8.0 h黏附细胞的吸光度。结果:红外光谱分析显示DP成功结合于PLGA膜表面,接触角测量表明PLGA膜经DP处理后亲水性改善,石英晶体损耗检验微量天平测得FN的负载量为22.5 ng/cm2,延时照相系统和CCK-8检测结果显示负载FN的PLGA膜上细胞黏附比例和数量显著高于PLGA膜和PLGA的DP涂层膜(P < 0.05)。结论:在PBS环境中,MC3T3-E1细胞可在负载FN的PLGA膜上正常黏附。

    Abstract:

    Objective:To prepare a fibronectin (FN) modified poly(lactic-co-glycolic acid) (PLGA) membrane via dopamine (DP), and evaluate the effects of FN on cell adhesion of ME3T3-E1. Methods: Attenuated total reflectance Fourier transform infrared spectrometer (FTIR), contact angle meter and quartz crystal microbalance with dissipation were performed to measure the surface groups, contact angle and FN loading of the samples; Time lapse imaging system was performed to observe cell adhesion of ME3T3-E1 every 3 min, and cell counting Kit-8 was used to measure the cell adhesion 0.5, 1.0, 4.0, 8.0 h after cell vaccination in phosphate buffered saline (PBS). Results: FTIR and contact angle showed DP was successfully grafted onto PLGA, and improved hydrophilicity was acquired on DP modified PLGA surfaces. Quartz crystal microbalance with dissipation showed that FN loading was 22.5 ng/cm2. Higher cell adhesion ratio was observed with time lapse imaging system and CCK-8 tests in FN loading samples than in PLGA and PLGA-DP membranes (P < 0.05). Conclusions: MC3T3-E1 can adhere to FN modified PLGA membranes via DP in PBS successfully.

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陈汉帮,陈 刚,李 娜,刘 珏,夏 阳,章非敏. PLGA经多巴胺改性后负载纤连蛋白对MC3T3-E1细胞黏附的影响[J].南京医科大学学报(自然科学版),2015,(1):31-35

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  • 收稿日期:2014-05-05
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  • 在线发布日期: 2015-02-06
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