文章摘要
环 飞,胡艳辉,秦 珩,张晓玲.雷公藤甲素对白血病HL-60和Jurkat细胞增殖及凋亡的影响[J].南京医科大学学报,2015,(1):036-039~50
雷公藤甲素对白血病HL-60和Jurkat细胞增殖及凋亡的影响
Effects of triptolide on the proliferation and apoptosis of human leukemia HL-60 and Jurkat cells
投稿时间:2014-07-21  
DOI:10.7655/NYDXBNS20150108
中文关键词: 雷公藤甲素  HL-60细胞  Jurkat细胞  细胞凋亡
英文关键词: triptrlide  HL-60 cell  Jurkat cell  apoptosis
基金项目:江苏高校优势学科建设工程项目
作者单位
环 飞 南京医科大学公共卫生学院,江苏省医药农药兽药安全性评价与研究中心,江苏 南京 211166 
胡艳辉 南京医科大学公共卫生学院,江苏省医药农药兽药安全性评价与研究中心,江苏 南京 211166 
秦 珩 南京医科大学公共卫生学院,江苏省医药农药兽药安全性评价与研究中心,江苏 南京 211166 
张晓玲 南京医科大学公共卫生学院,卫生检验学系,江苏 南京 211166 
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中文摘要:
      目的:探讨雷公藤甲素对人急性髓系白血病HL-60细胞?急性T淋巴细胞白血病Jurkat细胞增殖及凋亡的影响。方法:采用MTT法?Annexin V-FITC/PI双染法?PI染色法?透射电镜观察不同浓度雷公藤甲素作用于两种细胞,对其生长增殖?凋亡?细胞周期及形态等方面的影响。结果:雷公藤甲素能显著抑制HL-60?Jurkat细胞的增殖,降低其存活率。给药24 h后,半数细胞抑制剂量(IC50)分别为(42.50 ± 9.38)nmol/L和(60.91 ± 9.77)nmol/L。能以剂量依赖方式诱导两种细胞凋亡,细胞周期分布G1期比例增加,S期比例降低(P < 0.05),且伴有典型的细胞凋亡形态学改变。结论:雷公藤甲素能显著抑制HL-60?Jurkat细胞的生长增殖,并诱导细胞凋亡。
英文摘要:
      Objective:To determine the effect of triptolide on the proliferation and apoptosis of HL-60 cells in acute myeloid leukemia and Jurkat cells in T-cell acute lymphoblastic leukemia. Methods: Human leukemia cell lines were treated with triptolide at different concentrations. The inhibitory rate of cells proliferation was detected by MTT assay, apoptosis rate was detected by AnnexinV-FITC/PI double staining, cell cycle was observed by PI staining, and cell morphology was observed by transmission electron microscope (TEM). Results: Cell proliferation of HL-60 and Jurkat cells was significantly inhibited and their survival rates were reduced by triptolide in a dose-dependent manner, with the IC50 value for 24 h of (42.50 ± 9.38) nmol/L and (60.91 ± 9.77) nmol/L, respectively. Triptolide induced cells apoptosis in a dose-dependent manner,apparent cell cycle and typical apoptotic morphological changes. Conclusion: Triptolide could significantly inhibit cell proliferation and induce apoptosis in human leukemia cell lines of HL-60 and Jurkat.
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