Objective:To observe the effect of recombinant human Granulocyte/macrophage colonystimulating factor(rhGM-CSF) and nano-silver as treatment for deep burn degreen Ⅱ wound healing. Methods:The burn models were done with Wistar rats, which were randomly divided into three groups,petrolatum treatment (group A. n = 30),nano-silver treatment (group B. n = 30),and rhGM-CSF treatment(group C. n = 30). The healing rate of the three groups was recorded on postburn day 1,4,7,10,14,21. Meanwhile the levels of VEGF and EGF in serums were measured with ELISA. Results:Group A,group B,and group C were all neovascularization and epithelization on postburn day 10. The healing rate:group C>group B>group A. On postburn day 10,there were no significantly difference between groups (P > 0.05),but on postburn day 14,21,there were significantly statistical differences between groups(all P < 0.05). For VEGF levels,group A,group B,and group C were peaked on postburn day 21,14,14,respectively. On postburn day 1,there were no significantly statistical difference between groups (P > 0.05).On postburn day 4,7,10,14 and 21,there were significantly statistical difference between groups (P < 0.05). For EGF levels,All groups were peaked on postburn day 21. On postburn day 1 and 4,there were no significantly statistical differences between groups (P > 0.05). On postburn day 7 there wrer differences between C and group A and group B,and on day 10,14,21 differences were observed between groups (P < 0.05). Conclusions: rhGM-CSF and nano-silver treatment could promote wound healing,but rhGM-CSF is better than nano-silver.