文章摘要
杨景哲,陈凤平,冯欣姝,温海玲,耿琪瑛.rhGM-CSF、纳米银外用对深Ⅱ°烫伤创面愈合的影响研究[J].南京医科大学学报,2015,(2):192~195
rhGM-CSF、纳米银外用对深Ⅱ°烫伤创面愈合的影响研究
Effects of recombinant human Granulocyte/macrophage colonystimulating factor and nano-silver for deep burn degreen Ⅱ wound healing
投稿时间:2014-06-17  
DOI:10.7655/NYDXBNS20150211
中文关键词: 重组人粒细胞-巨噬细胞集落刺激因子  纳米银  烧伤  血管内皮生长因子  表皮生长因子
英文关键词: recombinant human granulocyte-macrophage colony stimulating factor(rhGM-CSF)  nano-silver  burn  VEGF  EGF
基金项目:承德市科学技术研究与发展计划项目(20122164);河北省医学科学研究重点课题计划指令性课题(20130024)
作者单位
杨景哲 承德医学院附属医院烧伤整形科,河北 承德 067000 
陈凤平 承德医学院附属医院烧伤整形科,河北 承德 067000 
冯欣姝 承德医学院附属医院烧伤整形科,河北 承德 067000 
温海玲 承德医学院附属医院烧伤整形科,河北 承德 067000 
耿琪瑛 承德医学院附属医院烧伤整形科,河北 承德 067000 
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中文摘要:
      目的:研究外用重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和纳米银,对深Ⅱ°烫伤创面愈合的影响。方法:用Wistar大鼠建立深Ⅱ°烫伤模型,分为A?B?C 3组,A组(30例):凡士林纱布覆盖;B组(30例):纳米银覆盖;C组(30例):rhGM-CSF涂抹创面;伤后第1?4?7?10?14?21天,观察创面愈合情况,计算愈合率,按照酶联免疫吸附法测定血清中血管内皮生长因子(VEGF)及表皮生长因子(EGF)水平。结果:A?B?C 3组均在伤后第10天出现血管化和上皮化;创面愈合率C组>B组>A组,第10天各组之间差异无统计学意义(P > 0.05),第14?21天各组之间差异有统计学意义(P < 0.05);VEGF水平,A组?B组?C组分别在第21?14天达峰值,伤后第1天各组间差异无统计学意义(P > 0.05),第4?7?10?14天各组间及第21天A组和C组之间差异有统计学意义(P < 0.05);EGF水平,各组均在第21天达峰值,第1?4天各组间及第7天A组与B组间差异无统计学意义(P > 0.05),第7天C组与A组?B组之间及第10?14?21天各组间差异均有统计学意义(P < 0.05)。结论:rhGM-CSF?纳米银外用,均加速深Ⅱ°烫伤创面愈合,并且rhGM-CSF效果优于纳米银。
英文摘要:
      Objective:To observe the effect of recombinant human Granulocyte/macrophage colonystimulating factor(rhGM-CSF) and nano-silver as treatment for deep burn degreen Ⅱ wound healing. Methods:The burn models were done with Wistar rats, which were randomly divided into three groups,petrolatum treatment (group A. n = 30),nano-silver treatment (group B. n = 30),and rhGM-CSF treatment(group C. n = 30). The healing rate of the three groups was recorded on postburn day 1,4,7,10,14,21. Meanwhile the levels of VEGF and EGF in serums were measured with ELISA. Results:Group A,group B,and group C were all neovascularization and epithelization on postburn day 10. The healing rate:group C>group B>group A. On postburn day 10,there were no significantly difference between groups (P > 0.05),but on postburn day 14,21,there were significantly statistical differences between groups(all P < 0.05). For VEGF levels,group A,group B,and group C were peaked on postburn day 21,14,14,respectively. On postburn day 1,there were no significantly statistical difference between groups (P > 0.05).On postburn day 4,7,10,14 and 21,there were significantly statistical difference between groups (P < 0.05). For EGF levels,All groups were peaked on postburn day 21. On postburn day 1 and 4,there were no significantly statistical differences between groups (P > 0.05). On postburn day 7 there wrer differences between C and group A and group B,and on day 10,14,21 differences were observed between groups (P < 0.05). Conclusions: rhGM-CSF and nano-silver treatment could promote wound healing,but rhGM-CSF is better than nano-silver.
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