文章摘要
杨 慧,许波进,郑旭琴,陈欢欢,崔 岱,段 宇.多氯联苯118引起大鼠甲状腺炎症反应的研究[J].南京医科大学学报,2015,(5):622~625
多氯联苯118引起大鼠甲状腺炎症反应的研究
A study of polychlorinated biphenyl 118 induced inflammation reaction in rat thyroid
投稿时间:2015-01-23  
DOI:10.7655/NYDXBNS20150505
中文关键词: 多氯联苯  甲状腺  炎症
英文关键词: polychlorinated biphenyl  thyroid  inflammation
基金项目:国家自然科学基金(81170726,81102032);江苏省医学重点人才资助(20111541);南京医科大学第一附属医院创新团队资助(20113012);江苏高校优势学科建设工程资助(PAPD)
作者单位
杨 慧 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
许波进 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
郑旭琴 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
陈欢欢 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
崔 岱 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
段 宇 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
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中文摘要:
      目的:探讨持续低浓度多氯联苯118(polychlorinated biphenyl 118,PCB118)暴露是否会引起大鼠甲状腺炎症反应。方法:①将40只清洁级成年雄性Wistar大鼠[体重(200 ± 10)g]随机分为4组,每组10只,分别给予溶剂对照玉米油及低?中?高剂量[10?100?1 000 μg/(kg·d)]的PCB118,每周腹腔注射5 d,每周称重,造模13周后取甲状腺组织进行HE染色,观察炎症细胞浸润情况;②CCK8实验用于检测PCB118对细胞活力的影响;③实时定量PCR(qRT-PCR)实验检测PCB118对炎症因子白细胞介素-6(IL-6)?肿瘤坏死因子-α(TNF-α)及细胞间黏附分子-1(ICAM-1)mRNA表达水平的影响。结果:①大鼠甲状腺组织HE染色可见各PCB118处理组有大量炎症细胞浸润; ②相对低浓度的PCB118(0~25 nmol/L)对细胞活力无显著影响(P > 0.05);③与对照组比较,不同低浓度的PCB118刺激后,IL-6?TNF-α及ICAM-1的mRNA表达量显著增加(P < 0.05)。结论:PCB118可引起大鼠甲状腺组织及甲状腺细胞发生炎症反应。
英文摘要:
      Objective:To explore whether polychlorinated biphenyl 118 (PCB118)could cause inflammation reaction in rat thyroid. Methods:①Forty male Wistar rats were randomly divided into 4 groups:solvent control group (corn oil),low dose,medium dose and high dose PCB118 groups,intraperitoneal injected with corn oil or different doses of PCB118 (10,100,1 000 μg/(kg?d),respectively)for 5 d per week,and weekly weighed. Thirteen weeks later,the thyroid tissues were stained using HE method and the infiltration of inflammatory cells were observed. ② The effects of PCB118 on FRTL-5 cells viability was assessed using a Cell Counting Kit-8 assay. ③Quantitative real-time PCR (qRT-PCR)was used to detect the mRNA expression of cytokines interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule-1 (ICAM-1 ). Results: ①In HE staining,there were a large number of inflammatory cells infiltration in PCB118-treated rat thyroid tissue; ②Relatively low concentrations of PCB118 had no significant effects on cells viability(P > 0.05); ③Compared to the control group,the mRNA levels of IL-6,TNF-α and ICAM-1 increased significantly(P < 0.05) after stimulating with different concentrations of PCB118. Conclusion:PCB118 could induce inflammation reaction in rat thyroid tissues.
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