Objective:To investigate the effects of over-expressed mitogen-inducible gene 6 (MIG6)on apoptosis,proliferation and invasion of endometrial carcinoma cell. Methods:Ishikawa cells were transfected with MIG6 over-expressed plasmid by Lipofectamine 2000,and quantitative RT-PCR and Western Blotting were adopted to detect the expression level of MIG6 before and after transfection. Cell apoptosis and proliferation were measured by flow cytometry and BrdU incorporation assay,respectively. Expression of cleaved caspase 3 and Cyclin D1 was determined by Western Blotting. Invasive potential of Ishikawa cells was tested using Transwell assay. Results:Both mRNA and protein levels of MIG6 in Ishikawa cells transfected with MIG6 over-expression plasmid were significantly higher than those of negative control group. Fold changes were 24.2 and 2.8,respectively. Up-regulated MIG6 expression for 48 h,the expression of cleaved caspase 3 was significantly elevated(P < 0.05),and the early apoptosis rate and late apoptosis rate was 1.7 times and 2.7 times more than that of negative control group(P < 0.01,P < 0.05); the expressions of cyclin D1 and phosphorylation of ERK were both down-regulated significantly(P < 0.05),BrdU incorporation rate decreased from 43.4 ± 2.4% to 30.5 ± 4.3%(P < 0.05); meanwhile,the amount of trans-membrane cells decreased from 36.2 ± 6.9 to 26.2 ± 3.8(P < 0.05). Conclusion:Up-regulation of MIG6 inhibits proliferation and invasion,while promotes apoptosis of endometrial carcinoma Ishikawa cells in vitro.