文章摘要
邢应如,孟阿娜,胡 东,张荣波.外周血中3种标记的Tregs细胞表达频率比较 邢应如1*,孟阿娜1,胡 东2,张荣波2[J].南京医科大学学报,2015,(6):798~803
外周血中3种标记的Tregs细胞表达频率比较 邢应如1*,孟阿娜1,胡 东2,张荣波2
Comparation of three methods for identifying Tregs of peripheral blood
投稿时间:2014-12-18  
DOI:10.7655/NYDXBNS20150608
中文关键词: 调节性T细胞  CD127  FoxP3  流式细胞仪
英文关键词: regulatory T cells  CD127  FoxP3  flow cytometry
基金项目:国家自然科学基金(81202294,81172778)
作者单位
邢应如 安徽理工大学附属东方肿瘤医院检验科,安徽 淮南 232001 
孟阿娜 安徽理工大学附属东方肿瘤医院检验科,安徽 淮南 232001 
胡 东 安徽理工大学医学院免疫与检验教研室,安徽 淮南 232001 
张荣波 安徽理工大学医学院免疫与检验教研室,安徽 淮南 232001 
摘要点击次数: 911
全文下载次数: 378
中文摘要:
      目的:分析CD4+CD25High?CD4+CD25+CD127Low/–?CD4+CD25+FoxP3+ 3种标记的Tregs细胞比例的相关性,探讨不同荧光素组合标记Tregs细胞的鉴定效果。方法:随机抽取健康体检者60例,平均分成2组(Ⅰ组?Ⅱ组),Ⅰ组用CD25-APC/CD127-PE/CD4-FITC 三色抗体标记,Ⅱ组用CD25-PE/CD127-Alexa Fluor■647/CD4-PerCP-Cy5.5标记;另选Ⅰ组10例,用CD25-FITC/FoxP3-PE/CD4-PerCP-Cy5.5/CD127-Alexa Fluor■647 四色抗体标记;流式细胞仪检测,CellQuest 软件分析,比较Tregs细胞结果差异,分析FoxP3+与CD127Low/–的相关性。结果:CD25+CD127Low/–Tregs细胞Ⅱ组(7.89 ± 1.37)%明显高于Ⅰ组(6.37 ± 1.83)%(P=0.001),2组中CD4+CD25High和CD4+CD25+CD127Low/–的相关系数(r)分别是0.944?0.916;(94.08 ± 1.93)%的CD25+FoxP3+细胞低表达CD127,CD25+FoxP3+和全血检测CD25+CD127Low/–的Tregs细胞有较高的相关性(r=0.846),结果差异不明显(P=0.774)。结论:CD25+CD127Low/–是Tregs细胞特异性标志,适合临床应用。全血检测Tregs细胞,用CD25-APC?CD127-PE?CD4-FITC荧光素组合检测结果与FoxP3相关度高。
英文摘要:
      Objective:To analyze the association of three kinds of Tregs labeled by CD4+CD25High, CD4+CD25+CD127Low/-, CD4+CD25+FoxP3+ and study the effect of different fluorescence labeling methods on Tregs identification. Methods: Sixty samples of the peripheral blood from 60 healthy people were randomly divided into 2 groups (group I and group Ⅱ). Group I was labeled by CD25-APC/CD127-PE/CD4-FITC, group Ⅱ was labeled by CD25-PE/CD127-Alexa Fluor■647/CD4-PerCP-Cy5.5. Another 10 samples from group I were labeled by CD25-FITC/FoxP3-PE/CD4-PerCP-Cy5.5/CD127-Alexa Fluor■647. All labeled samples were detected using flow cytometry, and the data were analyzed using CellQuest software to count the numbers of Tregs labeled with different methods, and then the relationships between them were analyzed. Results: CD25+CD127Low/- Tregs cells in group Ⅱ (7.89 ± 1.37)% was significantly higher than that in group Ⅰ (6.37 ± 1.83)%(P = 0.001). The correlation coefficient (r) of CD4+CD25High and CD4+CD25+CD127Low/- in the two group were 0.944 and 0.916, respectively;(94.08 ± 1.93)% of CD25+FoxP3+ cells were also labeled with low CD127 expression, there was a high correlation (r=0.846) and no significant difference (P=0.774)between them. Conclusion: CD25+CD127Low/- can specifically label Tregs, and is suitable for clinical application. Between the labellings with FoxP3 and CD25-APC/CD127-PE/CD4-FITC fluorescein combination, there is a high correlation to identify Tregs in the peripheral blood.
查看全文   查看/发表评论  下载PDF阅读器
关闭