文章摘要
吕 叶,茅卫东,苏 筠,沈孝兵,姜 藻.抵抗素样分子β在非小细胞肺癌中的表达及其与临床病理特征的关系[J].南京医科大学学报,2015,(6):823~826
抵抗素样分子β在非小细胞肺癌中的表达及其与临床病理特征的关系
Expression of RELMβ in non-small cell lung cancer and the its relationship with clinicopathological features
投稿时间:2015-01-19  
DOI:10.7655/NYDXBNS20150612
中文关键词: 抵抗素样分子β  非小细胞肺癌  基因表达
英文关键词: resistin-like molecule beta  non-small cell lung cancer  gene expression
基金项目:国家自然科学基金(81172619)
作者单位
吕 叶 东南大学附属中大医院肿瘤科,江苏 南京 210009东南大学附属江阴医院肿瘤科,江苏 江阴 214400 
茅卫东 东南大学附属江阴医院肿瘤科,江苏 江阴 214400 
苏 筠 东南大学附属江阴医院肿瘤科,江苏 江阴 214400 
沈孝兵 东南大学公共卫生学院,江苏 南京 210009 
姜 藻 东南大学附属中大医院肿瘤科,江苏 南京 210009 
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中文摘要:
      目的:探讨抵抗素样分子β(resistin-like molecule beta,RELMβ)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及其与临床病理特征的关系。方法:应用逆转录-聚合酶链反应(RT-PCR)方法和蛋白免疫印迹检测103例NSCLC组织及癌旁正常肺组织(其中65例具有配对的淋巴结转移标本)中RELMβ的表达情况,分析其表达与临床病理特征之间的关系。结果:RT-PCR和蛋白免疫印迹结果均显示,RELMβ在NSCLC组织中的表达明显高于正常肺组织。RELMβ mRNA在肺癌组织中的表达(68.93%)显著高于癌旁组织(23.33%,P < 0.05)。不同肿瘤的分化程度?TNM分期及淋巴结转移情况,RELM β的表达强度不同,差异有统计学意义(P < 0.05)。结论:RELM β在NSCLC组织中异常高表达,与肺癌临床病理学特征相关,可能促进NSCLC的恶性进程。
英文摘要:
      Objective:This study aimed to detect the expression of resistin-like molecule beta (RELMβ)in human non-small cell lung cancer (NSCLC) and its relationship with clinicopathological characteristics. Methods: We detected the expression levels of RELM β in 103 NSCLC tissues and the same patients’ adjacent noncancerous tissues (including 65 with paired lymph node metastases) by reverse transcription polymerase chain reaction (RT-PCR) and Western blot, and then analyzed their correlation with clinical pathological parameters. Results: RT-PCR and Western blot showed that the expression level of RELMβ in NSCLC tissues was significantly higher than that in noncancerous tissues. Compared with adjacent noncancerous tissues, the mRNA and protein expression levels of RELM β were significantly increased in lung cancer (23.33%,P < 0.05). The high expression of RELM β was closely associated with the degree of differentiation, TNM staging and lymph node metastasis (P < 0.05). Conclusion: The positive expression of RELM β is associated with clinical pathological parameters of NSCLC. RELM β may promote tumor progression.
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