文章摘要
周 萍,刘正霞.手足口病儿童血浆γ干扰素诱导蛋白10的表达水平及临床意义[J].南京医科大学学报,2015,(6):865~869
手足口病儿童血浆γ干扰素诱导蛋白10的表达水平及临床意义
Expression and clinical significance of interferon-γ-inducible protein-10 in the plasma of children with hand-foot-mouth disease
投稿时间:2014-09-15  
DOI:10.7655/NYDXBNS20150622
中文关键词: 手足口病  儿童  IP-10  淋巴细胞亚群
英文关键词: hand-foot-mouth disease  children  IP-10  lymphocyte subsets
基金项目:国家自然科学基金(81300999)
作者单位
周 萍 南京医科大学第二附属医院老年医学研究室,江苏 南京 210003 
刘正霞 南京医科大学第二附属医院老年医学研究室,江苏 南京 210003 
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中文摘要:
      目的:探讨γ干扰素诱导蛋白10(interferon-γ inducible protein-10,IP-10)在手足口病(hand-foot-mouth disease,HFMD)儿童血浆中的表达水平及临床意义。方法:选取HFMD患儿74例,根据是否伴有神经系统症状将患儿分为重症组和普通感染组;另选取30例体检儿童作为健康对照组。采用流式液相多重蛋白定量检测技术检测血浆中IP-10的表达量,同时检测分析淋巴细胞亚群比例?免疫球蛋白和血常规相关指标。比较各指标在健康对照组?HFMD普通感染组及重症组中的差异;分析IP-10与皮疹出现天数及淋巴细胞各亚群比例之间的相关性。结果:HFMD普通感染组及重症组患儿白细胞计数均增高,但和健康对照组相比无统计学差异。HFMD普通感染组及重症组患儿IP-10蛋白表达均显著增加(P < 0.001),重症组IP-10蛋白表达略低于普通感染组,差异无统计学意义(P > 0.05)。HFMD普通感染组及重症组患儿T细胞比例显著降低(P < 0.001),其中CD4+ T 淋巴细胞与CD8+ T 淋巴细胞亦显著降低(P < 0.05)。相反,B淋巴细胞比例显著增加(P < 0.001)。HFMD普通感染组?重症组与健康对照组相比,IgA降低,IgG增加,但无统计学差异(P > 0.05)。HFMD普通感染组患儿IP-10蛋白表达量与皮疹天数呈负相关(P < 0.05),而重症组虽IP-10蛋白表达量与皮疹天数呈负相关,但无统计学意义。HFMD重症组IP-10蛋白表达量与T淋巴细胞比例呈正相关,其中与CD4+ T 淋巴细胞的正相关有统计学意义(P < 0.01)。结论:HFMD患儿T淋巴细胞比例降低,B淋巴细胞比例增加,IP-10蛋白表达显著增加,与皮疹天数呈负相关。
英文摘要:
      Objective:To investigate the expression level and clinical significance of interferon-γ-inducible protein 10 (IP-10) in the plasma of children with hand-foot-mouth disease (HFMD). Methods:Seventy-four children with hand-foot-mouth disease and 30 healthy controls were enrolled in this study. The infectious cases were divided into severe group and common group,respectively, according to whether patients were accompanied with nervous system symptoms. The flow cytometry technology was performed to detect IP-10 expression in plasma. Meanwhile,the lymphocyte subsets ratio,immunoglobulin and routine blood tests were also detected. The indicators were compared among the healthy controls,the general infection group and the severe group. The correlation among IP-10,rash days and lymphocyte subsets ratios were analyzed. Results:The number of white blood cell increased in the common HFMD group and the severe group,but had no statistical difference compared to the healthy controls. The expression of IP-10 significantly increased in the disease groups (both P < 0.001); however,the expression of IP-10 in the severe group was slightly lower than that of the common group of infection (P > 0.05). T lymphocyte cell ratio decreased significantly in both two disease groups (P < 0.001),in which the CD4+ T lymphocyte cells and CD8+ T lymphocyte cells also obviously reduced (both P < 0.05). On the contrary,B lymphocyte cell proportion increased remarkably (P < 0.001). The IgA increased while IgG reduced in the infectious groups compared with the healthy controls without statistical difference (P > 0.05). The skin rash days was negatively correlated with IP-10 expression,notably in the general infection group (P < 0.05),but was no statistical difference compared to the severe group. The proportion of CD4+ T lymphocyte cells was significantly positively related to IP-10 expression in the severe group (P < 0.01). Conclusion:The expression of IP-10 and B lymphocyte cell ratio significantly increased and T lymphocyte cell ratio significantly decreased in children with HFMD,which were negatively correlated with skin rash days.
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