文章摘要
陈丽莎,徐永成,余志金,曾书君,罗 程,程进伟,陈惠新.JMJD2B通过ERK-MAPK途径影响人结直肠癌细胞的恶性表型[J].南京医科大学学报,2015,(7):962~967
JMJD2B通过ERK-MAPK途径影响人结直肠癌细胞的恶性表型
Effects of JMJD2B mediated ERK-MAPK signaling pathway in malignant phenotype of human colorectal cancer cells
投稿时间:2014-12-07  
DOI:10.7655/NYDXBNS20150712
中文关键词: 结直肠肿瘤  组蛋白去甲基化酶JMJD2B  细胞外信号调节MAP激酶类  细胞增殖
英文关键词: colorectal tumor  histone demethylase JMJD2B  extracellular-signal regulated MAP kinases  cellular proliferation
基金项目:广东省自然科学基金面上项目(S201310011923);惠州市科技计划项目(2013Y009)
作者单位
陈丽莎 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
徐永成 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
余志金 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
曾书君 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
罗 程 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
程进伟 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
陈惠新 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 
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中文摘要:
      目的:探讨组蛋白去甲基化酶JMJD2B影响人结直肠癌细胞恶性表型所介导的信号通路?方法:以RNA干扰技术靶向沉默人结直肠癌细胞HCT116和SW480中JMJD2B的表达,采用蛋白质印迹法检测人结直肠癌细胞ERK-MAPK信号通路的变化,并分别采用CCK-8?流式细胞分析检测细胞增殖和细胞周期分布?凋亡情况?结果:转染JMJD2B siRNA能特异性抑制JMJD2B的表达并导致ERK2表达下调,其磷酸化水平也降低,肿瘤细胞发生G2/M或G0/G1期阻滞,细胞凋亡比例增加,增殖显著受抑(P<0.05)?结论:抑制JMJD2B可通过阻断ERK-MAPK信号转导而抑制人结直肠癌细胞的恶性表型?
英文摘要:
      Objective:To investigate the signal pathway through which JMJD2B affected the malignant phenotype of human colorectal cancer cells. Methods: Human colorectal cancer cell lines HCT116 and SW1480 were interfered with siRNAs for silencing JMJD2B expression. The expression of ERK-MAPK signal pathway was detected by Western blotting, and cell proliferation was determined by CCK-8 assay, while cell cycle distribution and apoptosis were assessed by flow cytometry. Results: JMJD2B siRNA effectively and specifically inhibited the expression of JMJDB, which decreased ERK2 and its phosphorylation expression, and subsequently led to cell cycle arrest in G2/M or G0/G1 phase, increasing rates of cell apoptosis and significant cell proliferation inhibition (P < 0.05). Conclusion: Inhibition of histone demethylase JMJD2B restrained the malignant phenotype of human colorectal cancer cells via the inhibition of ERK-MAPK signal pathway transduction.
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