文章摘要
李赟颉,殷丽萍,陆 超.Par-4基因增敏顺铂对肾母细胞瘤荷瘤裸鼠生长的抑制作用[J].南京医科大学学报,2015,(9):1195~1199
Par-4基因增敏顺铂对肾母细胞瘤荷瘤裸鼠生长的抑制作用
Inhibitory effect on growth of xenograft with Wilms’ tumor by Par-4 gene sensitizing cisplatin
投稿时间:2015-04-07  
DOI:10.7655/NYDXBNS20150902
中文关键词: 肾母细胞瘤  Par-4基因  GRP78蛋白
英文关键词: nephroblastoma  Par-4 gene  GRP78 protein
基金项目:国家自然科学基金(81170487)
作者单位
李赟颉 南京医科大学第一附属医院儿科,江苏 南京 210029 
殷丽萍 南京医科大学第一附属医院儿科,江苏 南京 210029 
陆 超 南京医科大学第一附属医院儿科,江苏 南京 210029 
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中文摘要:
      目的:探讨腺病毒介导的前列腺凋亡反应因子-4(prostate apoptosis response-4,Par-4)协同顺铂(CDDP)对人肾母细胞瘤细胞SK-NEP-1裸鼠皮下移植瘤的影响。方法:采用人肾母细胞瘤细胞株SK-NEP-1建立BALB/c裸鼠皮下移植瘤模型,分别用表达Par-4的腺病毒?CDDP及两者联合治疗,随机分成5组:Par-4+CDDP组?Par-4组?CDDP组?空Par-4(不表达Par-4的对照腺病毒)组?空白对照组。测定移植瘤的体积及瘤重,通过HE染色及免疫组化分析,检测移植瘤组织中Par-4?GRP78及BAX蛋白的表达水平。结果:Par-4+CDDP组?Par-4组及CDDP组均有抑制裸鼠移植瘤生长的作用,瘤重差异有统计学意义(P < 0.05),其中,Par-4+CDDP组优于Par-4组与CDDP组。而空Par-4组与空白对照组相比,差异无统计学意义。Par-4+CDDP组能明显上调Par-4?GRP78及BAX蛋白表达(P < 0.05)。结论:外源性Par-4协同CDDP抑制裸鼠移植瘤的生长可能是外源性Par-4通过结合GRP78后上调BAX蛋白所致。
英文摘要:
      Objective:To study the effects of adenovirus mediated prostate apoptosis response-4 (Par-4)combined with cisplatin(CDDP)on subcutaneous xenografts of hunan Wilms’ tumor cells SK-NEP-1 in nude mouse. Methods:Inhibition effect of subcutaneous transplanted Wilms’ tumor model were established in BALB/c nude mice,and then the nude mice were randomly divided into five groups,namely Par-4+CDDP group,Par-4 group,CDDP group,negative control Par-4 group and blank control group. The volumes and weights of the transplanted tumors were measured. Transplanted tumor tissues were used to detect the expression levels of Par-4,GRP78 and BAX protein by HE staining and immunohistochemical analysis. Results:The tumor growth was inhibited in Par-4+CDDP group,Par-4 group and CDDP group,and there were differences in the tumor weight amorg 3 groups (P < 0.05). The inhibition effect of Par-4+CDDP group was superior to the Par-4 group and CDDP group. There was no statistically significace in tumor inhibition between blank control group and negative control Par-4 group. Par-4+CDDP can significantly increased Par-4,GRP78 and BAX protein(P < 0.05). Conclusion: Ectopic Par-4 in combination with CDDP suppressed the cancer-xenografted growth in nude mice,which might up-regulate BAX protein,the result of the interraction of extracellular Par-4 and GRP78.
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