文章摘要
黄文森,施亚雄,杨鑫娜,林莞蓉.血清PEDF及PEDF基因启动子区多态性与2型糖尿病患者微量白蛋白尿的相关性[J].南京医科大学学报,2015,(9):1216~1220
血清PEDF及PEDF基因启动子区多态性与2型糖尿病患者微量白蛋白尿的相关性
Association of serum pigment epithelium-derived factor and polymorphism in promoter region of pigment epithelium-derived factor gene with microalbuminuria in type 2 diabetes mellitus patients
投稿时间:2015-02-26  
DOI:10.7655/NYDXBNS20150906
中文关键词: 色素上皮衍生因子  单核苷酸多态性  2型糖尿病  微量白蛋白尿
英文关键词: pigment epithelium-derived factor  single nucleotide polymorphism  type 2 diabetes mellitus  microalbuminuria
基金项目:福建省教育厅科技项目(JA13396,JB13086)
作者单位
黄文森 泉州医学高等专科学校内科学教研室,福建 泉州 362000 
施亚雄 福建医科大学附属第二医院内分泌科,福建 泉州 362000 
杨鑫娜 福建医科大学附属第二医院内分泌科,福建 泉州 362000 
林莞蓉 福建医科大学附属第二医院内分泌科,福建 泉州 362000 
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中文摘要:
      目的:探讨福建地区汉族2型糖尿病患者血清色素上皮衍生因子水平(pigment epithelium-derived factor,PEDF)及PEDF基因启动子区rs1294385单核苷酸多态性与微量白蛋白尿的关系。方法:471例2型糖尿病患者根据尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR)分为正常组(UACR<30 μg/mg,NAU组)246例?微量白蛋白尿组(30 μg/mg≤UACR<300 μg/mg,MAU组)225例,使用PCR-RFLP方法检测PEDF基因启动子区rs1294385的多态性,同时检测血清PEDF水平及空腹血糖?空腹胰岛素?糖化血红蛋白?血脂等指标。结果:MAU组的血清PEDF水平高于NAU组,差异有统计学意义(P < 0.05);偏相关分析显示血清PEDF水平与UACR呈正相关(P < 0.05);PEDF基因启动子区rs1294385的基因型(GG型?GA型和AA型)和等位基因(G/A)在NAU?MAU两组间的分布存在差异,且差异有统计学意义(P < 0.05);GA基因型出现微量白蛋白尿的风险是GG基因型的1.838倍,GA+AA基因型出现微量白蛋白尿的风险是GG基因型的1.862倍,差异有统计学意义(P < 0.05)。结论:2型糖尿病患者血清PEDF水平随微量白蛋白尿的出现而增高;福建地区汉族2型糖尿病患者PEDF基因启动子区rs1294385多态性与微量白蛋白尿明显相关,携带A等位基因可能增加2型糖尿病患者发生微量白蛋白尿的机率。
英文摘要:
      Objective:To investigate the relationship between serum pigment epithelium-derived factor (PEDF) level,PEDF gene polymorphism in promoter region rs1294385 and microalbuminuria in type 2 diabetes mellitus (T2DM)of Fujian Han population. Methods:According to urinary albumin to creatinine ratio (UACR),471 T2DM patients were divided into the normal albuminuria group(the NAU group,UACR<30 μg/mg,n=246) and the microalbuminuria group(the MAU group,30 μg/mg≤UACR<300 μg/mg,n=225). The PEDF gene rs1294385 G→A polymorphism was measured based on polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Meanwhile,serum PEDF level,fasting plasma glucose(FPG),fasting insulin(FINS),glycosylated hemoglobin (HbA1c) and blood lipid were measured. Results:The level of serum PEDF in the MAU group was significantly higher than that in the NAU group(P < 0.05). According to partial correlation analysis,serum PEDF level was positively related with UACR(P < 0.05). There were significant differences between the frequencies of PEDF gene rs1294385G→A genotype (GG genotype,GA genotype and AA genotype)and allele(G/A)of the NAU group and those of the MAU group(P < 0.05). The risk ratio of GA genotype carriers suffering microalbuminuria was 1.838 times higher than that of GG genotype,while the risk ratio of GA and AA genotype carriers suffering microalbuminuria was 1.862 times higher than that of GG genotype(P < 0.05). Conclusion:Elevation of serum PEDF level is in accordance with the development of microalbuminuria in T2DM patients. Gene polymorphism in promoter region rs1294385 G→A of PEDF gene is closely related with microalbuminuria in T2DM patients of Fujian Han population. Moreover,T2DM patients with carriers of the A allele gene may have a higher risk of suffering from microalbuminuria.
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