文章摘要
孟 博,谢而付,周春刚.Tim-3在IgA肾病患者外周血单个核细胞中的表达及临床意义[J].南京医科大学学报,2015,(9):1225~1229
Tim-3在IgA肾病患者外周血单个核细胞中的表达及临床意义
Expression of human Tim-3 in PBMC from IgA nephropathy patients and its clinical significance
投稿时间:2015-03-25  
DOI:10.7655/NYDXBNS20150908
中文关键词: IgA肾病  免疫球蛋白黏蛋白3  mRNA
英文关键词: immunoglobulin A nephropathy  T cell immunoglobulin and mucin 3  mRNA
基金项目:国家自然科学基金(81101322)
作者单位
孟 博 南京中医药大学附属无锡中医院检验科,江苏 无锡 214071 
谢而付 南京医科大学第一附属医院检验学部,江苏 南京 210029 
周春刚 南京中医药大学附属无锡中医院中心实验室,江苏 无锡 214071 
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中文摘要:
      目的:探究免疫球蛋白黏蛋白3(T cell immunoglobulin and mucin 3,Tim-3)在IgA肾病(IgA nephropathy,IgAN)发病过程中的作用及其临床意义。方法:real-time qPCR检测41例IgAN患者及同期21例健康对照者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中Tim-3 mRNA表达水平。流式细胞术检测IgAN患者和健康对照者PBMC中Tim-3阳性细胞比例。记录肾组织活检前24 h尿蛋白定量?尿常规?尿沉渣?镜下红细胞数及血肌酐等临床相关指标。结果:Tim-3 mRNA在IgAN患者PBMC中表达明显升高(0.64 ± 0.27 vs. 0.18 ± 0.09,P < 0.01),且随疾病严重程度逐渐增高,Lee氏Ⅲ?Ⅳ?Ⅴ级患者Tim-3 mRNA水平明显高于Ⅰ和Ⅱ级患者,差异有统计学意义(P < 0.05);IgAN患者组PBMC中Tim-3阳性细胞比例显著高于对照组[(1.94 ± 0.77)% vs. (0.59 ± 0.24)%,P < 0.01],且随疾病严重程度存在上升趋势,Lee氏Ⅳ?Ⅴ级患者Tim-3阳性细胞比例高于Ⅰ?Ⅱ?Ⅲ级患者,差异有统计学意义(P < 0.05);Tim-3 mRNA和Tim-3阳性细胞比例与尿蛋白定量及血肌酐呈显著正相关。结论:Tim-3在IgAN患者中表达显著上调,并与24 h尿蛋白定量及血肌酐等临床指标相关,提示Tim-3可能参与IgAN的发生发展过程,并可能成为IgAN的潜在预后标志物。
英文摘要:
      Objective:The aim of this study was to investigate the expression of Tim-3 in pathogenesis of IgA nephropathy(IgAN)and its clinical significance. Methods:Real time qPCR was performed to detect Tim-3 mRNA in peripheral blood mononuclear cells(PBMCs)from 41 IgAN patients and 21 normal subjects. The proportion of Tim-3+ cells was assayed by flow cytometry. Results:24-hour proteinuria,serum creatinine,routine urine test,urine sediment examination and red blood cell count at high magnification were recorded before renal biopsy. Results:Tim-3 mRNA expression was increased significantly in IgAN patients compared with that in healthy controls(0.64 ± 0.27 vs. 0.18 ± 0.09,P < 0.01),and there was a nearly positive correlation between pathological grade and expression degree of Tim-3 mRNA. Levels of Tim-3 mRNA expression in patients with grade Ⅲ,Ⅳ and Ⅴ were higher than those in patients with grade Ⅰ and Ⅱ(P < 0.05). Also,we found that the proportion of Tim-3+ cells in PBMCs from IgAN patients was higher than that from normal controls[(1.94 ± 0.77)% vs. (0.59 ± 0.24)%,P < 0.01]. The proportion of Tim-3+ cells in patients with grade Ⅳ and Ⅴ was higher than that in patients with grade Ⅰ,Ⅱ and Ⅲ(P < 0.05). Moreover,the expression of Tim-3 mRNA and the proportion of Tim-3+ cells were significantly positively related to 24-hour proteinuria and serum creatinine. Conclusion:Up-regulation of Tim-3 in IgAN patients was positively correlated with some clinical indexes including 24-hour proteinuria and serum creatinine. Tim-3 may be involved in the pathogenesis of IgAN,and can be a reliable biomarker for evaluating disease severity and prognosis of IgAN.
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