文章摘要
董银凤,张 华.二甲双胍减轻氧糖剥夺/复糖复氧所诱导的小胶质细胞炎性反应[J].南京医科大学学报,2015,(10):1349~1354
二甲双胍减轻氧糖剥夺/复糖复氧所诱导的小胶质细胞炎性反应
Metformin reduces the oxygen-glucose deprivation and reoxygenation-induced microglial inflammation
投稿时间:2015-04-17  
DOI:10.7655/NYDXBNS20151003
中文关键词: 二甲双胍  氧糖剥夺  小胶质细胞  炎性反应
英文关键词: metformin  oxygen-glucose deprivation  microglia  inflammation
基金项目:江苏省高校自然面上项目(14KJB310010);南京中医药大学青年基金项目资助(13XZR30)
作者单位
董银凤 南京中医药大学护理学院内科学教研室,江苏 南京 210023 
张 华 南京中医药大学护理学院内科学教研室,江苏 南京 210024 
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中文摘要:
      目的:研究二甲双胍在氧糖剥夺/复糖复氧所致小胶质细胞炎性反应中的作用。方法:建立氧糖剥夺/复糖复氧模型诱导小胶质细胞(BV-2细胞株)的活化,给予不同浓度(0.02?0.20?2.00 mmol/L)的二甲双胍预处理BV-2细胞,通过MTT活性和乳酸脱氢酶活性检测确定二甲双胍的有效浓度;通过酶联免疫吸附试验测定细胞上清炎性因子白介素(IL)-1?茁的含量,免疫荧光检测细胞表面分子CD11b的表达及蛋白免疫印迹法分析胞核蛋白κB的表达。结果:二甲双胍(2.00 mmol/L)能够减少氧糖剥夺/复糖复氧模型诱导的BV-2细胞乳酸脱氢酶的释放并增加MTT的活性,降低细胞表面分子CD11b的表达,抑制胞核蛋白κB的核转位,减少IL-1?茁的生成与释放。结论:二甲双胍能够减轻氧糖剥夺/复糖复氧所致小胶质细胞的炎性反应。
英文摘要:
      Objective:To study the effects of metformin on oxygen glucose deprivation and reoxygenation (OGD/R)-induced microglial inflammatory reaction. Methods: To establish the OGD/R model of microglia (BV-2 cells), BV-2 cells were pretreated with different concentrations (0.02, 0.20 and 2.00 mmol/L) of metformin, the effective concentration of metformin was determined by MTT and lactate dehydrogenase (LDH) assays. The supernatant of IL-1?茁 was determined by enzyme-linked immunosorbent assay, expressions of nuclear factor kappaB and CD11b were analyzed by Western blot and immunofluorescence,respectively. Results: Metformin (2.00 mmol/L) decreased the release of OGD/R-induced LDH from BV-2 cells and increased MTT activity, reduced the expression of cell surface molecule CD11b, inhibited nuclear translocation of NF-kappaB, and decreased production and release of IL-1?茁. Conclusion: Pretreatment with metformin could reduce OGD/R-induced microglia inflammatory response.
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