文章摘要
刘 燕,冯旰珠,杜 强,杜兴冉,晋小祥.短期暴露于不同剂量细颗粒物对哮喘小鼠气道炎症的影响[J].南京医科大学学报,2015,(10):1364~1368
短期暴露于不同剂量细颗粒物对哮喘小鼠气道炎症的影响
Effects of fine particulate matter by different dosages at short-term exposure on airway inflammation in asthmatic mice
投稿时间:2015-02-27  
DOI:10.7655/NYDXBNS20151006
中文关键词: 哮喘  PM2.5  白细胞介素4  白细胞介素13
英文关键词: asthma  fine particulate matter  interleukin-4  interleukin-13
基金项目:国家自然科学基金(81470209);江苏省自然科学基金(BK20131436);南京医科大学科技发展基金(2014NJMU141)
作者单位
刘 燕 1南京医科大学第二附属医院呼吸科,江苏 南京 210011
2马鞍山市中心医院呼吸科,安徽 马鞍山 243000 
冯旰珠 南京医科大学第二附属医院呼吸科,江苏 南京 210011 
杜 强 南京医科大学第二附属医院呼吸科,江苏 南京 210011 
杜兴冉 南京医科大学第二附属医院呼吸科,江苏 南京 210011 
晋小祥 马鞍山市中心医院呼吸科,安徽 马鞍山 243000 
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中文摘要:
      目的:观察短期暴露于不同剂量细颗粒物(fine particulate matter,PM2.5)对哮喘小鼠气道炎症的影响,并初步探索PM2.5影响哮喘小鼠气道炎症机制。方法:将40只Balb/c小鼠采用随机数字表法分为5组:对照组?卵清蛋白(ovalbumin,OVA)组?OVA+低剂量PM2.5(10 ?滋g)组?OVA+中剂量PM2.5(31.6 ?滋g)组和OVA+高剂量PM2.5(100 ?滋g)组。通过腹腔注射OVA致敏?雾化吸入OVA构建小鼠哮喘模型,第26?28?30天予以PM2.5滴鼻激发。第31天处死小鼠后观察HE染色观察各组小鼠肺组织病理变化?炎性细胞浸润情况,比较各组小鼠肺泡灌洗液(BALF)中细胞总数及分类计数;并通过ELISA方法检测各组小鼠肺泡灌洗液中IL-3?IL-14及血清IgE水平。结果:OVA+低剂量PM2.5组小鼠BALF中细胞总数?分类计数百分比及IL-4?IL-13水平与OVA组相比均无统计学差异(P > 0.05);而OVA+中剂量PM2.5组及OVA+高剂量PM2.5组较OVA组升高(P < 0.05)。OVA+低剂量PM2.5组和OVA+中剂量PM2.5组血清IgE与OVA组相比轻度升高,但无统计学差异 (P > 0.05);OVA+高剂量PM2.5组较OVA组升高(P < 0.05)。结论:中剂量PM2.5(31.6 ?滋g)和高剂量PM2.5(100 ?滋g)可进一步加重哮喘小鼠气道炎症;而低剂量PM2.5(10 ?滋g)对哮喘小鼠气道炎症无显著影响。
英文摘要:
      Objective:To observe the effects of fine particulate matter (PM2.5) by different dosages at short-term exposure on airway inflammation in asthmatic mice, and study the mechanisms of PM2.5 on airway inflammation in asthmatic mice. Methods: A total of 40 BALB/c mice were randomly assigned into 5 groups, including the PBS control group, the OVA group (asthma group), the OVA PM2.5 (10 μg) group, the OVA PM2.5 (31.6 μg) group,and the OVA PM2.5 (100 μg) group. Asthma model was reproduced by sensitization with intraperitoneal injection and inhalation of OVA. On day 26, 28 and 30, the mice were performed with PM2.5 intranasal stimulation, and then sacrificed on day 31. We observed the pathological changes and inflammatory cell infiltration by hematoxylin-eosin (HE) staining of lung tissues of mice alveolar fluid (BALF) cells in each group. IL-4/IL-13 and serum IgE levels were examined by the method of ELISA. Results: Compared with the asthma group, the differences in cell count and levels of IL-4/IL-13 were not statistically significant in the PM2.5 (10 μg)/OVA group(P > 0.05), while those in the PM2.5(31.6 μg)/OVA group and the PM2.5(100 μg)/OVA group were increased (P < 0.05). The level of serum IgE in the PM2.5 (10 μg)/OVA group and the PM2.5 (31.6 μg)/OVA group was increased slightly compared with the OVA group, but there was no significant difference(P > 0.05). However, the level of serum IgE in the PM2.5(100 μg)/OVA group was significantly increased than that of the OVA group(P < 0.05). Conclusion: The asthma airway inflammation may further aggravate in the PM2.5(31.6 μg)/OVA and PM2.5 (100 μg)/OVA, and no significant effect was from the PM2.5 (10 μg)/OVA.
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