文章摘要
顾海燕,赵德育.维生素D缺乏对大鼠哮喘模型气道炎症的影响[J].南京医科大学学报,2015,(10):1369~1371
维生素D缺乏对大鼠哮喘模型气道炎症的影响
Effects of vitamin D deficiency on airway inflammation in asthmatic rats
投稿时间:2015-07-11  
DOI:10.7655/NYDXBNS20151007
中文关键词: 维生素D缺乏  哮喘  大鼠
英文关键词: vitamin D deficiency  asthma  rats
基金项目:南京医科大学科技发展基金面上项目(2010NJMU123)
作者单位
顾海燕 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210008 
赵德育 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210008 
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中文摘要:
      目的:研究维生素D(vitamin D,Vit D)缺乏对哮喘模型大鼠气道炎症的影响。方法:雄性SD大鼠16只(体重150 g左右),随机分为正常对照组?Vit D缺乏模型组,每组8只,对照组予正常光照及普通饲料喂养;模型组予以避光?不含Vit D的饲料喂养,4周后同时用卵清蛋白致敏和激发,建立哮喘动物模型。收集支气管肺泡灌洗液(bronchial alveolar lavage fluid,BALF)进行细胞计数;取肺组织石蜡包埋切片,观察气道炎症情况。结果:Vit D缺乏组的BALF中细胞总数及嗜酸性粒细胞百分比均明显高于对照组(P < 0.05);Vit D缺乏组的肺部病理改变程度较对照组更为严重。结论:Vit D缺乏加重哮喘大鼠气道炎症病变程度。
英文摘要:
      Objective:To study the effect of vitamin D deficiency on airway inflammation in rats with asthma. Methods:Sixteen Sprague-Dawley rats were divided into 2 groups randomly,including normal control group and vitamin D deficiency experiment model group,with 8 rats in each group. On days 1 and 7,the rats were sensitized with ovalbumin via introperitoneal injection and challenged with daily ovalbumin inhalation for 7 consecutive days since day 14. After the challenge,the bronchial alveolar lavage fluid (BALF) was collected for examining the total cell number and eosinophil count,and the lung tissue was taken to evaluate the airway inflammation. Results:Compared with the control group,the total BALF cell count and eosinophil percentage were found to increase in the vitamin D deficient model group (P < 0.05). More serious pathological changes and asthmatic airway inflammation were found in the lung tissues of rats lack of vitamin D. Conclution:Vitamin D deficiency could give rise to more frequent asthmatic attack and aggravate airway inflammation in asthmatic rats.
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