文章摘要
吴心池,王 尧,唐 伟,朱 琳,高 远.非增殖性糖尿病视网膜病变患者血清miR-29c的表达及相关分析[J].南京医科大学学报,2015,(10):1401~1404
非增殖性糖尿病视网膜病变患者血清miR-29c的表达及相关分析
Expression of serum miR-29c and its correlation analysis in patients with non-proliferative diabetic retinopathy
投稿时间:2015-06-06  
DOI:10.7655/NYDXBNS20151014
中文关键词: 微小RNA-29c  糖尿病视网膜病变  血清
英文关键词: microRNA  diabetic retinopathy  serum
基金项目:国家自然科学基金(81370920)
作者单位
吴心池 东南大学医学院附属江阴医院内分泌科,江苏 江阴 214400 
王 尧 东南大学附属中大医院内分泌科,江苏 南京 210009 
唐 伟 东南大学医学院附属江阴医院内分泌科,江苏 江阴 214400 
朱 琳 东南大学医学院附属江阴医院内分泌科,江苏 江阴 214400 
高 远 东南大学医学院附属江阴医院内分泌科,江苏 江阴 214400 
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中文摘要:
      目的:观察微小RNA-29c(miR-29c)在非增殖性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)患者血清中的表达水平,探讨其在糖尿病视网膜病变(diabetic retinopathy,DR)发病机制中的作用。方法:选择2型糖尿病NPDR患者及无视网膜病变(non-diabetic retinopathy,NDR)患者各30例。收集血清样本,应用荧光定量PCR方法检测血清miR-29c的相对表达水平。应用PicTar?TargetScan与MiRanda软件综合预测miR-29c的靶基因,取三款软件的交集作为最终靶基因。结果:miR-29c在NPDR患者血清中的表达量明显高于NDR组(P < 0.05),其表达量与HbA1c呈显著正相关(r=0.379,P < 0.05)。预测miR-29c靶基因集合富集在磷酸肌醇代谢?细胞因子及受体的相互作用?细胞外基质与受体信号作用通路中。结论:NPDR患者血清miR-29c表达显著上调,miR-29c可能通过调控细胞因子的相互作用?磷酸肌醇代谢等途径参与DR的发生发展。
英文摘要:
      Objective:To investigate the expression of serum miR-29c in the patients with non-proliferating diabetic retinopathy (NPDR) and explore the role of miR-29c in diabetic retinopathy (DR). Methods: Patients with NPDR (n=30) and without DR (n=30) were enrolled. Total RNAs including miRNA was extracted from serum samples. The levels of miR-29c were detected by quantitative Real-time PCR. PicTar, TargetScan and MiRanda were performed to comprehensively predict target genes of miR-29c, and the intersection of the three softwares was set as the final target genes. Results: The expression of serum miR-29c was significantly elevated in the NPDR group than that in the NDR group (P < 0.05). Positive correlation between miR-29c expression and HbA1c level (r=0.379, P < 0.05) was observed. The target genes of miR-29c were significantly enriched in inositol phosphate metabolism, interaction between cytokine and cytokine receptor, and ECM and receptor. Conclusion: The serum levels of miR-29c were significantly increased in the NPDR group. MiR-29c may play an important role in pathogenesis of DR by regulating cytokines interaction and inositol phosphate metabolism.
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