抑癌基因PTEN对人原代子宫内膜细胞凋亡及细胞周期的影响
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

南京市医学科技发展基金(YKK12104);南京医科大学科技发展基金(2011NJMU213)


Effect of tumor suppressor gene PTEN on human primary endometrial cell apoptosis and its cell cycle
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:通过人原代子宫内膜细胞实验研究第10号染色体缺失的磷酸酶(phosphatase and tensin homolog deleted on chromosome ten,PTEN)对子宫内膜异位症发生-发展的作用和意义。方法:利用慢病毒载体分别在人原代子宫内膜细胞中过表达和静默PTEN基因的表达;流式细胞仪检测慢病毒感染之后不同PTEN表达组的细胞周期及凋亡变化情况。结果:PTEN过表达组人原代子宫内膜细胞G0/G1期比例增加,与对照组比较差异具有统计学意义,G2/M期比例逐渐减少,细胞周期阻滞在G0/G1期;空白对照组-PTEN过表达组-PTEN静默组的细胞凋亡率分别是(11.70 ± 0.03)%-(15.80 ± 0.14)%-(5.33 ± 0.08)%。结论:PTEN可显著增加人原代子宫内膜细胞凋亡,抑制细胞周期,对子宫内膜异位症的发生发展有一定抑制作用。

    Abstract:

    Objective:To study the role and significance of phosphatase and tensin homolog deleted on chromosome ten(PTEN)protein for the occurrence and development of endometriosis (EMs)in vitro experiments. Methods:Lentiviral vector was used to overexpress PTEN and silence PTEN expression in human primary endometrial cells; flow cytometry was used to detect the changes in cell cycle and apoptosis in the different PTEN expression groups after lentivirus infection. Results:Cell cycle analysis showed that after transfection of overexpressed lentivirus of PTEN gene,the proportion of G0/G1 phase in human primary endometrial cells was increased,compared with the control group(P <0.05). The proportion of G2/M phase was gradually decreased,and cell cycle was arrested in G0/G1 phase; In the blank group,the overexpressed PTEN group,and the siPTEN group,cell apoptosis rates were(11.70 ± 0.03)%,(15.80 ± 0.14)%,and(5.33 ± 0.08)%,respectively. Conclusion:Overexpression of PTEN could significantly increase apoptosis rate and inhibit cell cycle of human primary endometrial cells. PTEN has a certain inhibiting effect on the occurrence and development of endometriosis.

    参考文献
    相似文献
    引证文献
引用本文

朱巧英,贾雪梅,陈 玲,余宁珠,吕 娟.抑癌基因PTEN对人原代子宫内膜细胞凋亡及细胞周期的影响[J].南京医科大学学报(自然科学版),2015,(11):1533-1537

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2015-08-04
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-11-30
  • 出版日期: