Objective:To investigate the application value of 99Tcm-DTPA renal dynamic imaging in the diagnosis and therapy of renal single mass. Methods:A total of 61 patients with renal mass were retrospectively collected from January 2010 to January 2015. All patients accepted 99Tcm-DTPA renal dynamic imaging and blood biochemical test while CT enhanced imaging and ultrasound examination were performed in most cases. Renal perfusion index (PI)and glomerular filtration rate (GFR)was evaluated by 99Tcm-DTPA renal dynamic imaging. 99Tcm-DTPA renal dynamic imaging was performed in 32 cases with renal malignant mass and 2 cases with benign tumor about 6 months later after operation. Results:The diagnostic sensitivity and accuracy of 99Tcm-DTPA renal dynamic imaging for renal malignant mass was 37.5% and 63.9%,respectively,lower than that of CT enhanced imaging,while the diagnostic sensitivity,specificity and accuracy was 66.7%,85.7% and 75.0%,respectively,when the long diameter of the renal mass was more than 4 cm. If diagnostic criteria were designed as PI ratio of the renal mass to normal contralateral kidney(T1/K1)was no more than 1,and GFR ratio of the renal mass of functional phase to contralateral(or adjacent)symmetrical kidney tissues(T2/K2)was almost 0,the diagnostic sensitivity increased to 83.3%. Nephron-sparing surgery was preferred when moderate-to-serious injury of the contralateral kidney was seen on 99Tcm-DTPA renal dynamic imaging. Six months after operation,the renal PI was decreased and GFR was increased,otherwise,the renal function inadequacy was found,some patients needed dialysis treatment. Conclusion:99Tcm-DTPA renal dynamic imaging,evaluating PI,GFR of the kidney and the renal mass,is useful for the diagnosis,operation guiding and prognosis prediction.