文章摘要
段 磊,杨 芳,何 闻,徐 璐,许 宁,刘 宾,顾 宁.聚合物微气泡的优化及其体内外超声成像研究[J].南京医科大学学报,2015,(12):1691~1696
聚合物微气泡的优化及其体内外超声成像研究
Optimized polymer microbubbles for ultrasound imaging in vitro/vivo
投稿时间:2015-06-13  
DOI:10.7655/NYDXBNS20151204
中文关键词: 微气泡  聚合物  优化  超声成像
英文关键词: microbubbles  polymer  optimization  ultrasound imaging
基金项目:国家自然科学基金青年基金(61401217);江苏省自然科学基金青年基金(BK20140900)
作者单位
段 磊 东南大学生物电子学国家重点实验室,江苏省生物材料与器件重点实验室,生物科学与医学工程学院,江苏 南京 210009
南京医科大学生物医学工程系,江苏 南京 210029 
杨 芳 东南大学生物电子学国家重点实验室,江苏省生物材料与器件重点实验室,生物科学与医学工程学院,江苏 南京 210009 
何 闻 东南大学生物电子学国家重点实验室,江苏省生物材料与器件重点实验室,生物科学与医学工程学院,江苏 南京 210009 
徐 璐 南京医科大学生物医学工程系,江苏 南京 210029 
许 宁 南京医科大学生物医学工程系,江苏 南京 210029 
刘 宾 南京医科大学生物医学工程系,江苏 南京 210029 
顾 宁 东南大学生物电子学国家重点实验室,江苏省生物材料与器件重点实验室,生物科学与医学工程学院,江苏 南京 210009 
摘要点击次数: 1481
全文下载次数: 998
中文摘要:
      目的:对以左旋聚乳酸(poly L-lactic acid,PLLA)及聚乙烯醇(polyvinyl alcohol,PVA)为膜材的微气泡,在稳定性和粒径两个方面进行了优化,使其在保持超声成像增强能力的同时,更适于体内应用。方法:首先对PVA进行了羧基化修饰,使制备的微气泡表面含有羧基,Zeta电位达到-38.87 ± 2.19 mV,从而由于静电斥力提高了微气泡的稳定性。其次,通过调节制备过程中的PVA浓度?水相油相比?乳化速度3种因素,实现了对微气泡粒径的控制。结果:对优化后平均粒径为1.38 ?滋m?粒径分布均匀?性质稳定的微气泡进行了体内外超声成像实验,证实其在体外能够显著增强超声成像效果,在体内既可以通过瘤内注射迅速提高肿瘤部位超声显影质量,又可以通过静脉注射,经血液循环到达肿瘤部位,在一段时间的累积之后,增强超声成像效果。结论:本研究为微气泡的体内应用打下了良好基础。
英文摘要:
      Objective:This study focuses on the optimization on stability and size of MBs with poly-L-lactic acid(poly L-lactic acid,PLLA)and polyvinyl alcohol (polyvinyl alcohol,PVA)as shell materials,which made MBs more suitable to work in vivo at the same time keeping the ability of enhancing the ultrasound imaging. Methods:First,PVA was modified to obtain telechelic PVA with carboxylic groups at the chain ends. The modified PVA was used to fabricate MBs present carboxyl on their surface,whose Zeta potential was -38.87 ± 2.19 mV,thus improving the stability of MBs. Next,The particle size and stability of MBs were regulated through adjustments the three factors,i.e. the concentration of PVA aqueous solution,the water-oil ratio of the first emulsification and the rate of second emulsification during the preparation process. Results:In vitro/vivo US imaging experiment was performed on the optimized MBs,which had a average particle size of 1.38 ?滋m,uniform particle size distribution and good stability. In vitro experiments showed that the MBs exhibited significantly ultrasound(US)contrast enhancement. In vivo US imaging of tumors in nude mice showed that,by intratumoral injection,MBs can rapidly improve the ultrasound imaging of tumor;by intravenous injection,MBs can reach the tumor site through the blood circulation,accumulate in the tumor site after a period of time,and enhance ultrasound imaging. Conclusion:This study lays a good foundation for the application of MBs in vivo.
查看全文   查看/发表评论  下载PDF阅读器