文章摘要
王钟林,张冬生,王 勇,孙跃明.miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响[J].南京医科大学学报,2015,(12):1703~1706
miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响
The effects of miRNA145 combined with metformin on the proliferation of human HCT116 cancer cells
投稿时间:2015-09-12  
DOI:10.7655/NYDXBNS20151206
中文关键词: miRNA145  二甲双胍  结直肠癌  增殖
英文关键词: miRNA145  metformin  colorectal cancer  proliferation
基金项目:江苏省六大人才高峰项目(2013)
作者单位
王钟林 南京医科大学第一附属医院普外科,江苏 南京 210029
连云港市第二人民医院普外科,江苏 连云港 222000 
张冬生 南京医科大学第一附属医院普外科,江苏 南京 210029 
王 勇 南京医科大学第一附属医院普外科,江苏 南京 210029 
孙跃明 南京医科大学第一附属医院普外科,江苏 南京 210029 
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中文摘要:
      目的:探讨miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响。方法:慢病毒法将miRNA145转染入人结直肠癌细胞株HCT116,实时定量PCR检测细胞miRNA145表达的变化。通过CCK-8细胞增殖实验检测miRNA145和/或二甲双胍对HCT116细胞增殖的影响。通过Western blot检测miRNA145和/或二甲双胍对细胞AMPK?mTOR蛋白表达水平的影响。结果:CCK-8实验表明miRNA145?二甲双胍均具有抑制HCT116细胞增殖的作用,二者联用使细胞增殖受到更大程度的抑制。Western blot结果表明,二甲双胍?miRNA145以及二者联用可进一步促进AMPK的活化进而影响mTOR蛋白表达水平。结论:miRNA145联合应用二甲双胍具有更强的抑制人结肠癌细胞株HCT116增殖的作用。
英文摘要:
      Objective:To investigate the effects of miRNA145 combined with metformin on the proliferation of human HCT116 cancer cells. Methods:miRNA145 overexpression mediated by recombinant lentivirus was exployed in HCT116 cells. Cell proliferation assay by CCK-8 method was conducted to determine the roles of miRNA145 and/or metformin on the cancer cells. Western blot analysis was used to determine the expression levels of AMPK and mTOR proteins. Results:Both miRNA 145 and metformin can inhibit the cell proliferation of HCT116. The combination of miRNA 145 and metformin exerted a much stronger inhibitory effect on cell proliferation than any of the two. The activation level of AMPK was also higher by the combination,and the p-mTOR expression level was lower by the combination. Conclusion:miRNA145 and metformin can exert an anticancer effect synergistically.
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