文章摘要
王亚勤,刘 翔,吴 晨,唐义虎,吴延虎.c-Myc基因在人非小细胞肺癌中的表达及基因沉默抑制H1299细胞效果的实验研究[J].南京医科大学学报,2016,(2):151~154
c-Myc基因在人非小细胞肺癌中的表达及基因沉默抑制H1299细胞效果的实验研究
The expression of c-Myc in non-small cell lung cancer and the effect of its silencing in inhibiting tumor cells
投稿时间:2015-06-13  
DOI:10.7655/NYDXBNS20160205
中文关键词: 非小细胞肺癌  基因治疗  c-Myc基因
英文关键词: non-small-cell lung carcinoma  gene therapy  c-Myc gene
基金项目:江苏省六大人才高峰(2012-WS-108)
作者单位
王亚勤 南京医科大学第一附属医院胸心外科,江苏 南京 210029 
刘 翔 南京医科大学第一附属医院胸心外科,江苏 南京 210029 
吴 晨 常州市第一人民医院肿瘤中心,江苏 常州 213000 
唐义虎 南京医科大学第一附属医院胸心外科,江苏 南京 210029 
吴延虎 南京医科大学第一附属医院胸心外科,江苏 南京 210029 
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中文摘要:
      目的:分析人非小细胞肺癌(non-small cell carcinoma,NSCLC)组织中c-Myc基因的表达,探讨沉默c-Myc基因在非小细胞肺癌基因治疗中的前景?方法:应用实时荧光定量PCR法检测84例非小细胞肺癌组织及其相应癌旁组织中c-Myc基因mRNA的表达情况?通过脂质体LipofactAMINE2000将c-Myc基因siRNA瞬时转染至非小细胞肺癌H1299癌株细胞中,顺铂(Cisplatin,cis)药物毒性实验验证c-Myc基因沉默对肿瘤细胞的抑制效果?应用RT-PCR检测H1299细胞中c-Myc基因RNA的表达,CCK-8法检测抑制细胞的效果?结果:非小细胞肺癌组织中c-Myc基因mRNA的表达水平高于相应的癌旁组织,差异有统计学意义(P < 0.05)?c-Myc siRNA瞬时转染后的H1299细胞中,c-Myc基因mRNA的表达水平显著下降(P < 0.05)?将c-Myc基因沉默和顺铂联合使用,还可以显著提高顺铂对H1299细胞增殖的抑制效果(P < 0.05)?结论:c-Myc基因在非小细胞肺癌组织中的表达水平明显高于相应癌旁组织,沉默c-Myc基因能显著提高顺铂抑制非小细胞肺癌H1299 细胞增殖的作用,c-Myc基因有可能成为NSCLC基因治疗的新靶点?
英文摘要:
      Objective:To investigate the expression of c-Myc in non-small cell lung cancer (NSCLC)tissues,and to explore the prospects of the c-Myc gene silencing in NSCLC gene therapy. Methods:The expression levels of c-Myc mRNA in 84 NSCLC tissues and the corresponding para-cancerous tissues were detected by RFQ-PCR(real-time fluorescence quantitative-PCR). C-Myc siRNA was transfected into NSCLC H1299 cells by LipofectAMINE 2000. Cisplatin toxicity experiments further validated effect of inhibiting tumor cells with c-Myc gene silencing. The expression level of c-Myc mRNA in H1299 cells was detected by RT-PCR,and the effect of inhibiting H1299 cells was detected by CCK-8 (Cell Counting Kit-8). Results:The expression level of c-Myc mRNA in NSCLC tissues was higher than that in the corresponding para-cancerous tissues (P < 0.05). C-Myc siRNA was successfully transfected into NSCLC H1299 cells. After transfection,the expression level of c-Myc mRNA was down-regulated in H1299 cells. Effect of inhibiting tumor cells increased significantly when the c-Myc gene silencing and cisplatin were in combination (P < 0.05). Conclusion:The expression level of c-Myc mRNA in NSCLC tissues is higher than that in the corresponding para-cancerous tissues. The lower expression of c-Myc can significantly inhibit NSCLC H1299 cells. It is suggested that c-Myc may act as an oncogene in NSCLC gene therapy.
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