文章摘要
杨玉青,卢新政,曹佳莉,邹花一阳,朱丽红,张 辉,孙慧文,陈明龙,孔祥清.左西孟旦对去甲肾上腺素诱导的心肌细胞钙瞬变信号的影响[J].南京医科大学学报,2016,(2):166~170
左西孟旦对去甲肾上腺素诱导的心肌细胞钙瞬变信号的影响
Effects of levosimendan on calcium transient in norepinephrine-cultured neonatal rat ventricular myocytes
投稿时间:2015-07-03  
DOI:10.7655/NYDXBNS20160208
中文关键词: 左西孟旦  去甲肾上腺素  乳鼠心肌细胞  钙瞬变信号
英文关键词: levosimendan  norepinephrine  the neonatal rat ventricular myocyte  calcium transient
基金项目:江苏省“六大人才高峰”项目(No2015-WSN-004)
作者单位
杨玉青 南京医科大学第一附属医院心内科,江苏 南京 210029 
卢新政 南京医科大学第一附属医院心内科,江苏 南京 210029 
曹佳莉 南京医科大学第一附属医院心内科,江苏 南京 210029 
邹花一阳 南京医科大学第一附属医院心内科,江苏 南京 210029 
朱丽红 南京医科大学第一附属医院心内科,江苏 南京 210029 
张 辉 南京医科大学第一附属医院心内科,江苏 南京 210029 
孙慧文 南京医科大学第一附属医院心内科,江苏 南京 210029 
陈明龙 南京医科大学第一附属医院心内科,江苏 南京 210029 
孔祥清 南京医科大学第一附属医院心内科,江苏 南京 210029 
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中文摘要:
      目的:研究心肌细胞衰竭过程中钙瞬变信号的变化及左西孟旦(levosimendan,LeV)对其影响?方法:培养Sprague Dawley(SD)乳大鼠心肌细胞48 h后,分为对照组?去甲肾上腺素(NE)组?NE+LeV1(0.1 μmol/L)诱导组和NE+LeV2(1 μmol/L)诱导组?NE组以去甲肾上腺素10 μmmol/L诱导;NE+LeV1组先用NE诱导,再以LeV 0.1 μmol/L干预;NE+LeV2组先用NE诱导,再以LeV 1 μmol/L干预?用Ca2+荧光指示剂Fluo-3/AM负载心肌细胞,激光共聚焦显微镜观察心肌细胞钙瞬变信号变化以及心肌细胞的搏动频率?结果:① 与对照组相比,NE组心室肌细胞钙波分散?传导减慢?同步性较差,细胞搏动频率较快(26.7 ± 4.3 vs. 11.6 ± 3.6,P < 0.01)?收缩期钙瞬变峰值下降(128.37 ± 65.44 vs. 155.33 ± 61.77,P < 0.05)?达峰时间(Ttp,0.413 ± 0.324 vs. 0.212 ± 0.050,P < 0.01)?衰减时间(Tau,1.162 ± 0.524 vs. 0.722 ± 0.169,P < 0.01)均延长,钙瞬变幅度(60.80 ± 39.88 vs. 75.41 ± 36.52,P > 0.05)?谷值(67.57 ± 42.59 vs. 79.92 ± 38.05,P > 0.05)变化不大;② LeV处理后,钙波传导均匀同步,心肌细胞搏动频率及钙瞬变的峰值?幅度?谷值较NE组无明显变化,NE+LeV1组和NE+LeV2组Ttp分别为 (0.212 ± 0.044?0.205 ± 0.062)?Tau分别为(0.735 ± 0.269?0.753 ± 0.152)均较NE组缩短(P < 0.01);③NE+LeV1与 NE+LeV2两组相比,钙瞬变峰值和谷值?钙瞬变幅度?Ttp和Tau差别无统计学意义?结论:心肌细胞衰竭过程中钙瞬变减弱变慢?同步性较差;LeV通过增加钙瞬变速度?改善钙瞬变传导形式,发挥正性肌力作用?
英文摘要:
      Objective:To explore the alterations of the calcium transient in the development of heart failure,and the impact of levosimendan(LeV)on intracellular Ca2+ dynamics. Methods:The neonatal rat ventricular myocytes in culture were divided into 4 groups,normal,NE alone (10 μmol/L),NE+LeV1(0.1 μmmol/L), and NE+LeV2(1 μmmol/L). Calcium transients of myocytes loaded by Fluo-3/AM were observed under Laser scanning confocal microscope. Results:(1)Compared with the control,calcium wave dispersed,conducted slowly,and exibited dyssynchrony of Ca2+ release in NE group,NE accelerated the cultured cell beating rate(26.7 ± 4.3 vs. 11.6 ± 3.6,P < 0.01),decreased peak systolic Ca2+(128.37 ± 65.44 vs. 155.33 ± 61.77,P < 0.05),shortened time to peak(Ttp;0.413 ± 0.324 vs. 0.212 ± 0.050,P < 0.01)and decay time(Tau;1.162 ± 0.524 vs. 0.722 ± 0.169,P < 0.01)of calcium transient,except Ca2+ transient amplitude and resting Ca2+.(2)NE in the presence of LeV increased synchrony of calcium transient,reduced Ttp(0.212 ± 0.044 vs. 0.413 ± 0.324,NE+LeV1 vs. NE,P < 0.01 and 0.205 ± 0.062 vs. 0.413 ± 0.324,NE +LeV2 vs. NE,P < 0.01 respectively)and Tau (0.735 ± 0.269 vs. 1.162 ± 0.524,NE +LeV1 vs. NE,P < 0.01; and 0.753 ± 0.152 vs. 1.162 ± 0.524,NE + LeV2 vs. NE,P < 0.01),had no effect on cell beating rate,peak systolic or resting Ca2+,and transient amplitude.(3)There were no concentration difference in the presence of Levosimendan. Conclusion:Our results show that calcium transient gets weaker and slower,exibiting dyssynchrony of Ca2+ release in failing rat ventricular myocyte. Levosimendan exerts positive inotropic effect through accelerating the velocity of calcium signaling propagation and synchronzing the calcium release.
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