Objective:We sought to construct a full human anti-Trop-2 IgG expression vector,and to express and purify it,then analyze the effect of anti-Trop-2 IgG in human ovarian cancer cells. Methods:The recombinant expression vector of full human anti-Trop-2 IgG was constructed and the IgG was expressed in 293 FreeStyle (293F)cell eukaryotic expression system and then purified using AKTA system. The immune activity of the IgG was verified and analyzed by SDS-PAGE,enzyme-linked immunosorbent assay(ELISA),affinity assay,Western blot assay,flow cytometry method (FCM)and immunofluorescence assay. The effect on the character of ovarian cancer cells was detected by cell counting kit-8 (CCK-8)assay,wound healing assay,transwell test and cell apoptosis assay. Results:The results demonstrated that the full human anti-Trop-2 IgG was successfully produced. The IgG specifically bound Trop-2 protein,and it could play an important role in the proliferation,migration and invasion of ovarian cancer cells as well as in inducing ovarian cancer cell apoptosis. When the concentration of IgG was at 400 μg/mL,only 55.95%(P < 0.01)of HO8910 cells survived. The amount of invasive cells in the experimental group was only 32.11% of those in the control group(P < 0.05). The wound healing rate of the experimental group was 24.71% while that of control group being 70.80%(P < 0.01),and the apoptosis rate was 2-folds higher than the control group (P < 0.05). Conclusion:The full human anti-Trop-2 IgG could specifically bind Trop-2 protein on the surface of ovarian cancer cells and restrain the malignant biological behavior of ovarian cancer cells. The IgG has potential applications in ovarian cancer targeting therapy.