文章摘要
孙 进,李凤飞,朱红红,李建民,马建华.胰岛β细胞中PP2ACα基因失活小鼠模型的建立及初步表型分析[J].南京医科大学学报,2016,(4):416~419
胰岛β细胞中PP2ACα基因失活小鼠模型的建立及初步表型分析
Establishment and preliminary phenotypic analysis of mouse model of PP2AC specifical inactivation in pancreatic-cells
投稿时间:2015-08-04  
DOI:10.7655/NYDXBNS20160407
中文关键词: PP2ACα  小鼠模型  糖耐量
英文关键词: PP2AC  mouse model  glucose tolerance
基金项目:中国博士后科学基金资助(2015M581829)
作者单位
孙 进 南京医科大学附属南京医院内分泌科,江苏 南京 210006 
李凤飞 南京医科大学附属南京医院内分泌科,江苏 南京 210006 
朱红红 南京医科大学附属南京医院内分泌科,江苏 南京 210006 
李建民 南京医科大学江苏省医药动物实验基地,江苏 南京 211166 
马建华 南京医科大学附属南京医院内分泌科,江苏 南京 210006 
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中文摘要:
      目的:建立胰岛β细胞特异性失活 PPACα基因小鼠模型,对其表型进行初步观察。方法:通过 Ins2-Cre小鼠与 PP2ACαflox/flox 小鼠交配,获得胰岛β细胞特异性失活 PP2ACα基因小鼠(PP2ACαflox/fIox:Ins2-Cre)。PCR 和 Western blot 鉴定 PP2ACα基因第二外显子敲除小鼠(KO)。4 月龄时行腹腔注射葡萄糖耐量试验(IPGTT),以 PP2ACαflox/flox小鼠为对照。结果:①PP2ACα转录本在 KO 小鼠短于对照小鼠;②KO 小鼠胰岛中 PP2AC 蛋白水平较对照小鼠显著下降(P < 0.05);③IPGTT 结果显示:4月龄 KO 小鼠 30?60?120 min 时血糖明显高于对照小鼠(P < 0.05)。结论:成功建立胰岛β细胞特异性失活 PPACα基因小鼠模型,4月龄 PP2ACαflox/fIox:Ins2-Cre小鼠糖耐量受损。
英文摘要:
      Objective:To establish mouse model of PP2AC gene specifical inactivation in pancreatic cells and investigate preliminary animal phenotype. Methods: The Ins-2 transgenic mice in which Cre enzyme was exclusively detected in pancreatic-cells bred with PP2ACαflox/flox mice to obtain PP2ACαflox/+:Ins-2 mice, then bred with the PP2ACαflox/flox mice again to obtain PP2ACαflox/flox:Ins-2 mice (KO mice). We identified the 2nd exon of PP2ACα knockdown by PCR and Western Blot. Interperitoneal glucose tolerance test (IPGTT) was performed in transgenic mice at 4 months, and PP2ACαflox/flox mice were employed with control. Results: PP2ACα transcripts were shorter in KO mice than those in control mice. The protein level of PP2AC expression in KO mice was significantly lower than that of control mice (P < 0.05). The results of IPGTT indicated that the blood glucose levels at 30 min, 60 min and 120 min were significantly higher than those of controls (P < 0.05). Conclusion: Specifical inactivation of PP2AC in pancreatic β-cells mouse model is successfully established, and glucose tolerance impairs at 4 months in PP2ACαflox/flox:Ins2-Cre mice.
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