门脉高压性胃病大鼠VEGF及其受体FLT-1和FLK-1的表达
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南京市卫生局课题(2012YKK12080)


Expression of vascular endothelial growth factor and it’s receptors FLT-1 and FLK-1 in rats with portal hypertensive gastropathy
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    摘要:

    目的:探讨门脉高压性胃病(portal hypertensive gastropathy,PHG)模型大鼠胃组织血管内皮生长因子(VEGF)及其受体FLT-1和FLK-1的表达情况-方法:Wistar大鼠60只随机分为PHG组及假手术(SO)组,一期缩窄门静脉主干法制作PHG大鼠模型,术后2周用生理测压仪测定门静脉压力,免疫组化法测定大鼠胃组织VEGF及其受体的表达,并利用RT-PCR法测定VEGF及其受体mRNA的表达-结果:PHG组大鼠门静脉压力显著高于SO组(P < 0.05),免疫组化结果显示,PHG组大鼠胃组织细胞胞浆中VEGF及其受体的阳性表达率明显高于SO组(P < 0.05);RT-PCR结果显示,PHG组VEGF及其受体mRNA的表达显著高于SO组(P < 0.05)-结论:VEGF及其受体FLT-1和FLK-1可能参与PHG的形成-

    Abstract:

    Objective:To study the expression of vascular endothelial growth factor(VEGF) and it’s receptors FLT-1 and FLK-1 in rats with portal hypertensive gastropathy. Methods: Wistar rats were devided into two groups:PHG group whose portal hypertension was established by ligating the portal vein,and the sham-operation(SO)group served as control. After two weeks portal vein pressure was measured by physiological pressure capsule. Immunhistochemistry was used to detect the expression of VEGF and it’s receptors,and RT-PCR was used to detect the mRNA expression of VEGF and the receptors in both groups. Results: The pressure of PHG group was higher significantly than that in SO group(P < 0.05). And the PHG group has a significantly higher expressions of VEGF and the receptors compared with SO group(P < 0.05),the same of mRNA expressions. Conclusion: VEGF and the receptors may participate in the pathogenesis of portal hypertensive gastropathy.

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陶 平,黄 冠,潘 超,戴树龙,王小平.门脉高压性胃病大鼠VEGF及其受体FLT-1和FLK-1的表达[J].南京医科大学学报(自然科学版),2016,(5):589-592

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  • 收稿日期:2015-06-17
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  • 在线发布日期: 2016-05-23
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