文章摘要
章建飞,曹文达,汪 璟,王 清,鲁晓杰.人白细胞介素-33在胶质瘤中高表达并能促进胶质瘤细胞U251的侵袭作用[J].南京医科大学学报,2016,(6):682~686
人白细胞介素-33在胶质瘤中高表达并能促进胶质瘤细胞U251的侵袭作用
IL-33 expression is increased in human glioma and promotes U251 glioma cells invasion
投稿时间:2015-11-13  
DOI:10.7655/NYDXBNS20160608
中文关键词: 人白细胞介素-33  胶质瘤细胞  侵袭
英文关键词: human interleukin-33  glioma cells  invasion
基金项目:无锡市医管中心重大项目(YGZXZ1406)
作者单位
章建飞 南京医科大学附属无锡二院神经外科,江苏 无锡 214002 
曹文达 南京医科大学附属无锡二院神经外科,江苏 无锡 214003 
汪 璟 南京医科大学附属无锡二院神经外科,江苏 无锡 214004 
王 清 南京医科大学附属无锡二院神经外科,江苏 无锡 214005 
鲁晓杰 南京医科大学附属无锡二院神经外科,江苏 无锡 214006 
摘要点击次数: 618
全文下载次数: 314
中文摘要:
      目的:探讨人白细胞介素(interleukin,IL)-33在胶质瘤中的表达及在胶质瘤细胞U251侵袭中的作用?方法:采用免疫组化法检测了16例正常脑组织及86例不同级别胶质瘤标本IL-33蛋白的表达情况;应用Transwell小室检测IL-33对U251侵袭的影响;应用Western blot检测IL-33对胶质瘤细胞NF-κB及其磷酸化状态的影响?结果:免疫组化结果显示,IL-33在胶质瘤组织中表达高于正常脑组织(54.65% vs. 6.25%,P < 0.001)?IL-33在低级别胶质瘤(WHO Ⅰ?Ⅱ级)和高级别胶质瘤(WHO Ⅲ?Ⅳ级)组织中的阳性表达率分别为17.4%和68.3%(P < 0.001)?体外胶质瘤细胞的功能试验中,IL-33能促进U251细胞的侵袭(P < 0.05),并伴随NF-κB蛋白表达上调(P < 0.05)?结论:IL-33在胶质瘤中高表达,IL-33可能通过促进胶质瘤细胞NF-κB磷酸化,从而促进胶质瘤细胞的侵袭能力,对IL-33作用机制的进一步研究将为胶质瘤的临床治疗提供新思路?
英文摘要:
      Objective:To detect the expression of human interleukin-33 (IL-33) in glioma and study its effects on invasion of human U251 glioma cells. Methods:Immunohistochemistry was performed to detect IL-33 protein expression in 16 normal brain tissues and 86 patients with different grades of gliomas. Transwell assay was conducted to study the effect of IL-33 on invasion of U251 glioma cells. Western blotting assays were performed to investigate changes in NF-κB expression and its phosphorylation status after IL-33 treatment. Results:IL-33 protein expression was significantly higher in glioma tissues compared with normal brain tissues (54.65% vs 6.25%,P < 0.001). The expression of IL-33 in low-grade (WHO Ⅰ and Ⅱ) glioma and high-grade(WHO Ⅲ and Ⅳ) glioma was 17.4% and 68.3% (P < 0.001),respectively. IL-33 enhanced U251 cellular invasion (P < 0.05) in vitro,and the protein expression of NF-κB was increased (P < 0.05). Conclusion:IL-33 is frequently overexpressed in glioma tissues,and may contribute to glioma cell invasion by up-regulation of NF-κB phosphorylation.
查看全文   查看/发表评论  下载PDF阅读器
关闭