文章摘要
林加娟,白 咪,庄一波,宋瑞花,张园园,张爱华,黄松明,丁桂霞.PEA3在白蛋白诱导肾小管上皮细胞损伤中的作用[J].南京医科大学学报,2016,(8):923~927
PEA3在白蛋白诱导肾小管上皮细胞损伤中的作用
Role of PEA3 in the injury of renal proximal tubular cells induced by albumin
投稿时间:2016-02-13  
DOI:10.7655/NYDXBNS20160805
中文关键词: PEA3  白蛋白  肾近端小管上皮细胞  凋亡  上皮-间充质转分化
英文关键词: PEA3  albumin  renal proximal tubular cells  apoptosis  epithelial-mesenchymal transition
基金项目:国家自然科学基金资助(81070551)
作者单位
林加娟 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
白 咪 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
庄一波 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
宋瑞花 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
张园园 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
张爱华 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
黄松明 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
丁桂霞 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
摘要点击次数: 766
全文下载次数: 473
中文摘要:
      目的:探讨PEA3在白蛋白诱导的肾近端小管上皮细胞损伤中的作用及机制?方法:体外培养小鼠近端肾小管上皮细胞,实验组分为对照组?空载对照组?PEA3过表达组?白蛋白造模组和PEA3处理组?实时荧光定量PCR检测PEA3转染效率?应用Annexin V-FITC/PI双染和Hoechst染色检测细胞凋亡?Western blot法和RT-PCR检测肾小管上皮细胞标志物E-cadherin?α-SMA及Vimentin的蛋白和mRNA变化水平? 结果:①PEA3质粒转染后,过表达组的PEA3表达水平增高了2.5倍;②加入白蛋白刺激后,与对照组相比小管细胞凋亡增加,过表达PEA3显著抑制白蛋白诱导的小管细胞凋亡;③白蛋白刺激降低E-cadherin 的表达,而增加了α-SMA和Vimentin的表达,PEA3过表达阻断白蛋白对E-cadherin的抑制作用,并降低α-SMA和Vimentin 的表达?结论:PEA3可抑制白蛋白诱导的小管上皮细胞凋亡和上皮-间充质转分化?
英文摘要:
      Objective:To investigate the role of PEA3 in albumin induced mouse proximal tubular cells(mPTCs)apoptosis and its underlying mechanism. Methods:The cells were cultured with albumin in vitro,and divided into the control group,the vehicle control group,the PEA3 overexpression group,the albumin model group and the PEA3 overexpression in albumin model group. Real-time fluorescence quantitative polymerase chain reaction was used to investigate the level of PEA3 gene transfection efficiency. Flow cytometry and Hoechst staining technique were performed to analyze the apoptosis of mPTCs. The mRNA and protein levels of E-cadherin,α-SMA and Vimentin were detected by RT-PCR and Western bolt. Results:Transfection of PEA3 over-expresed the mRNA level of PEA3 for 2.5 fold. The apoptosis of mPTCs treated with albumin was increased compared with the control group,and overexpression of PEA3 ameliorated albumin-induced apoptosis. Albumin treatment reduced the expression of E-cadherin and increased the expression of Vimentin and α-SMA. Overexpression of PEA3 reversed the reduction of E-cadherin and ameliorated the increase of Vimentin and α-SMA. Conclusion:Overexpression of PEA3 can protect mPTCs from apoptosis and epithelial-mesenchymal transition induced by albumin.
查看全文   查看/发表评论  下载PDF阅读器
关闭