文章摘要
陈 浩,林燕蝶,邹怡新,李 皓.短期生酮饮食对小鼠代谢的影响[J].南京医科大学学报,2016,(8):937~941
短期生酮饮食对小鼠代谢的影响
Short-term effect of ketogenic diet on metabolism of mice
投稿时间:2016-02-23  
DOI:10.7655/NYDXBNS20160808
中文关键词: 生酮饮食  血糖  脂肪酸氧化
英文关键词: ketogenic diet  blood glucose  fatty acid oxidation
基金项目:国家自然科学基金(81070678);南京医科大学2014年大学生创新训练计划项目
作者单位
陈 浩 南京医科大学病理生理学系,江苏 南京 211166 
林燕蝶 南京医科大学病理生理学系,江苏 南京 211166 
邹怡新 南京医科大学病理生理学系,江苏 南京 211166 
李 皓 南京医科大学病理生理学系,江苏 南京 211166 
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中文摘要:
      目的:探讨短期生酮饮食对小鼠能量代谢的影响?方法:将Balb/c 小鼠随机分成2组,分别给予普通饮食或生酮饮食4 d,每天称量体重并检测血糖变化?在第4天,取血检测甘油三酯(TG)?总胆固醇(TC)?游离脂肪酸(FFA)?丙氨酸氨基转移酶(ALT)?天门冬氨酸氨基转移酶(AST)?β-羟丁酸(β-HB)水平;称量肝脏重量,测定肝脏中TG?胆固醇以及糖原含量;real-time PCR检测肝脏组织中代谢相关基因的表达水平;制作肝脏HE染色切片,显微镜下观察肝脏病理学改变?结果:短期生酮饮食使小鼠体重明显减轻,血糖明显下降,血TC?β-HB?FFA高于普通饮食小鼠?生酮饮食小鼠肝重/体重比增加,肝组织中的TG增多,糖原减少?real-time PCR显示生酮饮食组小鼠脂肪酸氧化?氧化磷酸化以及酮体生成相关基因增加?显微镜下可见生酮饮食小鼠肝脏脂质蓄积明显增加?结论:生酮饮食可快速促进小鼠脂肪酸氧化和酮体生成?与长期生酮饮食不同的是,短期生酮饮食引起血TC显著增加和脂质合成基因FAS表达增多,而对肝脏无明显损伤作用?
英文摘要:
      Objective:To investigate short-term effect of ketogenic diet on metabolism of mice. Methods:BALB/c mice were divided into two groups and placed on normal diet or ketogenic diet for 4 days. Body weight and blood glucose were monitored everyday. On the fourth day,the blood samples and livers were gathered and liver weight/body weight were calculated. Serum triglycerides(TG),total cholesterol(TC),free fatty acids(FFA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),and beta hydroxybutyric acid(β-HB)were tested. The content of TG,cholesterol and glycogen in liver were detected. The expression levels of genes related to metabolism in livers were detected by real-time PCR. Liver pathology was also examined by HE staining. Results:Short-term ketogenic diet significantly reduced blood glucose and body weight of mice. Liver weight/body weight of short-term ketogenic mice was higher than that of control mice. A four-day feeding of ketogenic diet resulted in increased TC and β-HB in blood. Meanwhile,more TG and less glycogen were detected in the liver. Real-time PCR demonstrated that hepatic genes involved in fatty acid oxidation,oxidative phosphorylation and ketogenesis were increased. Accumulation of lipid was observed in the liver of ketogenic mice under a microscope. Conclusion:Short-term ketogenic diet induced fatty acid oxidation and ketogenesis in mice. Different from long-term ketogenic mice,elevated blood cholesterol and FAS mRNA levels were observed in short-term ketogenic mice without obvious liver damage.
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