文章摘要
马 腾,赵 晔,邵永丰.LncRNA-HOTTIP 对食管鳞癌细胞增殖和侵袭的影响[J].南京医科大学学报,2016,(8):947~951
LncRNA-HOTTIP 对食管鳞癌细胞增殖和侵袭的影响
Silence of LncRNA-HOTTIP inhibits cell proliferation and invasion in esophageal squamous cell cancer
投稿时间:2016-03-13  
DOI:10.7655/NYDXBNS20160810
中文关键词: 食管鳞癌  长链非编码RNA-HOTTIP  细胞增殖  细胞侵袭
英文关键词: esophageal squamous cell cancer  long noncoding RNA HOTTIP  proliferation  invasion
基金项目:江苏省自然科学基金(BK20151590);江苏省六大人才高峰项目(WSW-024)
作者单位
马 腾 南京医科大学第一附属医院心脏大血管外科,江苏 南京 210029
东南大学附属中大医院胸心外科 
赵 晔 消化内科,江苏 南京 210009 
邵永丰 南京医科大学第一附属医院心脏大血管外科,江苏 南京 210029 
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中文摘要:
      目的:探讨HOXA 远端转录本(HOXA transcript at the distal tip,HOTTIP)在食管鳞癌(esophageal squamous cell cancer,ESCC)组织中的表达及对食管癌细胞部分生物学行为的影响?方法:采用逆转录-实时定量PCR法(RT-qPCR)检测ESCC组织及细胞中HOTTIP的表达水平;通过RNA干扰技术将si-HOTTIP转入细胞中以降低HOTTIP的表达水平,并通过定量PCR检测其干扰效率?用CCK8法和Transwell法检测敲除HOTTIP对细胞增殖能力和侵袭能力的影响?结果:与癌旁组织相比,ESCC组织中HOTTIP的表达明显升高;与正常食管上皮细胞相比,ESCC细胞中HOTTIP的表达明显升高?此外,通过分析临床数据发现高表达的HOTTIP与肿瘤体积和淋巴结转移紧密相关?CCK8和transwell实验显示,在敲除HOTTIP后,Eca-109和TE-13细胞的增殖和侵袭能力明显下降?结论:HOTTIP在ESCC细胞的增殖和侵袭过程中具有相当重要的作用?
英文摘要:
      Objective:To detect the expression level of HOXA transcript at the distal tip (HOTTIP)in esophageal squamous cell cancer(ESCC)tissues and cell lines and investigate the potential underlying molecular mechanism. Methods:qRT-PCR was used to determine the relative HOTTIP expression levels in ESCC tissues and cell lines. Small interfering RNA(si-HOTTIP)was transfected into ESCC cells to knock down HOTTIP expression. CCK-8 assay and Transwell assay were performed to evaluate the proliferation and invasion ability of ESCC cells. Results:Compared with controls,HOTTIP expression levels were significantly up-regulated in ESCC tissues and cell lines. Higher expression level of HOTTIP was found associated with larger tumor size and more lymph node metastasis. CCK-8 assay and Transwell assay showed that silence of HOTTIP inhibited the proliferation and invasion of Eca-109 and TE-13 cells in vitro. Conclusion:HOTTIP plays crucial role in proliferation and invasion of ESCC.
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