文章摘要
张园园,王 璐,苗 莉,宋瑞花,林加娟,白 咪,张爱华,黄松明.SRT1720对DOCA盐敏感性高血压大鼠的治疗作用[J].南京医科大学学报,2016,(8):960~963
SRT1720对DOCA盐敏感性高血压大鼠的治疗作用
Therapeutic effect of SRT1720 on DOCA salt-sensitive hypertensive rats
投稿时间:2016-03-27  
DOI:10.7655/NYDXBNS20160813
中文关键词: SRT1720  高血压  醋酸去氧皮质酮
英文关键词: SRT1720  hypertension  deoxycorticosterone acetate
基金项目:国家973项目(2013CB530604)
作者单位
张园园 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
王 璐 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
苗 莉 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
宋瑞花 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
林加娟 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
白 咪 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
张爱华 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
黄松明 南京医科大学附属南京儿童医院肾脏科,江苏 南京 210008 
摘要点击次数: 547
全文下载次数: 365
中文摘要:
      目的:观察沉默信息调节因子2相关酶1(Sirtuin1,SIRT1)激活剂SRT1720对醋酸去氧皮质酮(DOCA)盐敏感性高血压大鼠(DHR)血压的调控作用?方法:28只SD大鼠行左肾切除后随机分为3组:对照组?模型组?SRT1720治疗组?对照组(n=8):饮自来水;模型组(n=10)和SRT1720治疗组(n=10):将200 mg/kg DOCA与硅胶制成的橡皮介质放置在大鼠两侧肩胛骨之间的皮下,并予1% NaCl饮水?SRT1720治疗组给予100 mg/(kg·d)灌胃?每周测收缩压1次?4周后处死,处死前检测中心动脉压及大鼠脉搏波速度,处死后取胸主动脉分别作HE染色观察主动脉厚度与血管口径变化?结果:模型组大鼠收缩压?中心动脉压?脉搏波速度明显增高,HE染色示血管平滑肌细胞(VSMC)明显肥大,弹力纤维层增厚,主动脉厚度显著增厚,而血管内径则明显下降?SRT1720治疗组大鼠收缩压?中心动脉压及脉搏波速度均较模型组显著下降?同时,SRT1720治疗后血管壁的病理改变明显减轻,血管平滑肌细胞肥大受到显著抑制,主动脉厚度显著下降,而血管内径明显增加?结论:SRT1720可通过抑制血管平滑肌细胞肥大,降低主动脉厚度,从而扩大血管内径,降低中心动脉压及收缩压?
英文摘要:
      Objective:To investigate the role of silent mating type information regulation 2 homolong1(SIRT1)activator SRT1720 in deoxycorticosterone acetate(DOCA)salt-sensitive hypertensive rats(DHR). Methods:A total of 28 male Sprague-Dawley rats underwent left nephrectomy were randomly divided into three groups:control group,model group,and SRT1720 treatment group. Control group(n=8):drinking water; model group(n=10)and SRT1720 treatment group (n=10):DOCA and silicone rubber medium at the concentration of 200 mg/kg was placed subcutaneously in rats on both sides of the scapula and given 1% NaCl drinking water. SRT1720 treatment group was given intragastric administration of SRT1720 for 4 weeks at the concentration of 100 mg/(kg·d). Systolic blood pressure(SBP)was measured once a week. After 4 weeks,central arterial pressure and pulse wave velocity (PWV)were monitored,and then rats were sacrificed. Thoracia aorta was taken for HE staining to observe the thickness and vascular caliber change. Results:In model group,SBP,central aortic pressure and PWV were significantly increased. HE staining showed marked vascular smooth muscle cell(VSMC)hypertrophy,and marked thickening of elastic fiber layer and aortic media,but a significantly decreasing in vessel diameter. Meanwhile,SRT1720 treatment group reversed these changes. Conclusion:SRT1720 could inhibit VSMC hypertrophy,reduce aortic media thickness,thereby expend blood vessel diameter,and reduce central arterial pressure and peripheral blood pressure in DOCA salt-sensitive hypertensive rats.
查看全文   查看/发表评论  下载PDF阅读器
关闭