GIT1通过Notch信号通路促进骨髓细胞向破骨细胞分化
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国家自然科学基金(81271988)


GIT1 regulates differentiation of bone marrow cells into osteoclasts through Notch signaling pathway
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    摘要:

    目的:研究G蛋白偶联受体激酶结合蛋白1(G-protein coupled receptor kinase-interacting protein 1,GIT1)是否通过Notch信号通路影响骨髓细胞向破骨细胞分化。方法:取8周龄GIT1野生型(GIT1+/+)和GIT1基因敲除(GIT1-/-)小鼠各8只,分离培养小鼠骨髓细胞并向破骨细胞诱导分化。分别在诱导后的4-7 d,检测细胞中抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)活性。在诱导后4-7 d,用real-time RT-PCR检测破骨细胞相关基因组织蛋白酶K(cathepsin K,CTSK)-降钙素受体(calcitonin receptor,CTR)和基质金属蛋白酶9(matrix matalloproteinase,MMP-9)的表达,同时检测Notch信号通路受体-配体和目的基因的表达。结果:小鼠骨髓细胞向破骨细胞诱导分化过程中,基因敲除组破骨细胞的大小-数量-密度均低于野生型组;基因敲除组破骨细胞CTSK-CTR和MMP-9的表达均明显低于野生型组,差异有统计学意义 (P < 0.05);基因敲除组Notch信号通路配体Jagged1-受体Notch2和目的基因Hes1的表达明显高于野生型组,差异有统计学意义(P < 0.05)。结论:GIT1缺失可能通过上调Notch信号通路的表达来抑制骨髓细胞向破骨细胞分化。

    Abstract:

    Objective:To research whether G-protein coupled receptor kinase-interacting protein 1(GIT1) regulates the differentiation of bone marrow cells into osteoclasts through Notch signaling pathway. Methods:Bone marrow cells, which obtained from eight GIT1+/+ and eight GIT1-/- mice of 8-week-old, were isolated and cultured,then were induced to osteoclast differentiation. The tartrate-resistant acid phosphatase (TRAP) activity was detected to evaluate the osteoclast differentiation at day 4 and 7. Real-time RT-PCR were performed to detect the expression of cathepsin K (CTSK),calcitionin receptor(CTR),matrix metalloproteinase 9 (MMP9) and Notch signaling pathway at day 4 and 7. Results: The size,number and density of osteoclasts were lower in the GIT1 gene knockout group compared with the wild type group. The expression of CTSK,CTR and MMP9 in GIT1 gene knockout group was significantly lower than that of the wild type group (P < 0.05). However,The expression of Jagged1,Notch2 and Hes1 in the GIT1 gene knockout group was significantly higher than that of the wild type group (P < 0.05). Conclusion:The deficiency of GIT1 inhibited the differentiation of bone marrow cells into osteoclasts through upregulating Notch signaling pathway.

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恽 波,周灵杰,凡 进,殷国勇. GIT1通过Notch信号通路促进骨髓细胞向破骨细胞分化[J].南京医科大学学报(自然科学版),2016,(9):1036-1040

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  • 收稿日期:2016-03-13
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  • 在线发布日期: 2016-09-18
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