Objective:To study the endostatin(ES) gene armed endothelial progenitor cells(EPCs) for liver carcinoma H22 of mice and to discuss the feasibility and effectiveness of autologous EPSs combined with ES for treawent of liver carcinoma. Methods:We construct a lentiviral vector expressing endostatin gene and cultured the bone-marrow derived EPCs. Cell surface markers were detected with qPCR and cells were observed by electron microscope. There were three groups:EPCs,EPCs+LV,and EPCs+ES. Cell enhancement was detected by CCK-8 assay in the three groups. Liver carcinoma models in situ were made and three groups of cells were injected through tail veins. Mice were sacrificed later at different times and the tumors were detected. Results:The construction of lentiviral vectors pLenti6.3-ES-Monomer-DsRed was confirmed by cleavage,sequence identification and PCR. The cultured cells expressed endothelial cells’ markers CD31,VEGFR and VWF with qPCR. Characteristic WP globule for endothelial cell was observed by transmission electron microscope. EPCs transfected with ES were observed red. Supernatant in the group of EPC+ES could inhibit the enhancement of H22 than the group of EPC and EPC+LV. In vivo assay showed that the tumors of EPCs+ES were smaller than the other two groups. Conclusion:Mice bone marrow derived MNCs can be induced to EPCs,EPCs transfected with ES could supress the enhancement of H22 in vitro and the liver carcinoma in vivo.