文章摘要
陆沁源,邵东华.右美托咪定预处理对缺血再灌注损伤大鼠心肌线粒体功能的影响[J].南京医科大学学报,2016,(10):1198~1201
右美托咪定预处理对缺血再灌注损伤大鼠心肌线粒体功能的影响
Effects of dexmedetomidine preconditioning on mitochondrial function in rats with myocardial ischemia reperfusion injury
投稿时间:2016-04-22  
DOI:10.7655/NYDXBNS20161010
中文关键词: 右美托咪定  心肌缺血再灌注损伤  线粒体
英文关键词: dexmedetomidine  myocardial ischemia reperfusion injury  mitochondria
基金项目:镇江市社会发展基金(Sh2014087)
作者单位
陆沁源 江苏大学附属人民医院麻醉科,江苏 镇江 212002 
邵东华 江苏大学附属人民医院麻醉科,江苏 镇江 212002 
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中文摘要:
      目的:观察右美托咪定预处理对心肌缺血再灌注损伤(myocardial ischemia reperfusion injury,MIRI)大鼠线粒体功能的影响,探讨其对缺血再灌注心肌的保护作用。方法:将30只健康雄性SD大鼠随机分为3组:假手术组(A),MIRI组(B),MIRI+右美托咪定组(C)。采用结扎冠脉左前降支的方法建立心肌缺血再灌注损伤大鼠模型,通过心脏超声检测大鼠心功能的变化,real-time PCR检测线粒体功能相关基因mRNA水平的表达。透射电镜观察心肌线粒体超微结构。结果:与A组相比,B组和C组心肌功能受损,线粒体功能相关基因mRNA表达均降低(P < 0.05);与B组相比,C组心肌功能损伤减轻,线粒体功能相关基因mRNA表达水平明显增加(P < 0.05)。与A组相比,B组线粒体超微结构损伤显著;与B组相比,C组线粒体损伤程度明显减轻。结论:右美托咪定预处理可以减轻缺血再灌注所致心肌线粒体功能损伤,改善心肌功能,发挥保护心肌作用。
英文摘要:
      Objective:To investigate the effects of dexmedetomidine preconditioning on mitochondrial function of myocardial ischemia reperfusion injury(MIRI),and to explore the protective effect of dexmedetomidine preconditioning on myocardium with ischemia and reperfusion injure. Methods:A total of 30 healthy male Sprague Dawley(SD)rats,aged 8 weeks,were randomly divided into three groups:the sham operation group(group A),the myocardial ischemia-reperfusion group(group B),and the dexmedetomidine preconditioning group(group C). The model of myocardial ischemia reperfusion injury was performed by ligation of the left anterior descending coronary artery. Cardiac functions were detected by echocardiography. The expression of the mRNA levels associated with mitochondrial function was performed by real-time PCR. Ultrastructure of myocardial mitochondria was observed under electronic microscope. Results:Compared with group A,the cardiac function of group B and group C were markedly decreased;the mRNA levels of genes related to mitochondrial function were significantly lower in group B and group C than that in group A(P <0. 05). Compared to group B,the cardiac function of group C was enhanced;the mRNA levels of genes related to mitochondrial function were higher in group C than in group B(P < 0.05). We also found that impaired mitochondrial ultrastructure was observed in group B. Compared with group B,impaired mitochondrial structure was significantly improved in group C. Conclusion:Dexmedetomidine preconditioning could improve mitochondrial function to alleviate myocardial ischemia reperfusion injury.
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