牙龈卟啉单胞菌定植对钛表面氧化膜特性的影响
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国家自然科学基金(81472928);江苏省自然科学基金(BK20130898);江苏省野六大人才高峰冶资助(2014-WSW-035);江苏省高校大学生创新计划项目(201310312020Z);江苏高校优势学科建设工程资助(2014-37)


Effects of Porphyromonas gingivalis colonization on titanium surface oxide film properties
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    目的:研究牙龈卟啉单胞菌定植对钛表面氧化膜特性的影响。方法:将纯钛试件浸泡于牙龈卟啉单胞菌菌悬液中进行共培养,采用扫描电镜观察钛表面牙龈卟啉单胞菌生物膜的微结构。以牙龈卟啉单胞菌菌悬液为实验组,生理盐水为对照组,收集7-14-21 d的试件和浸泡液,采用X射线光电子能谱(XPS)分析浸泡后纯钛的表面特性,采用电感耦合-等离子体发射光谱仪(ICP-OES)检测不同时间点浸泡液中的钛离子释放量。结果:扫描电镜观察到牙龈卟啉单胞菌在钛表面定植形成致密的生物膜。XPS广谱分析显示,钛表面的钛和氧元素含量随细菌作用时间延长逐步降低;XPS高像素谱分析显示,钛表面的二氧化钛含量随细菌作用时间延长逐步降低。ICP-OES检测发现随着细菌作用时间的延长,钛离子释放量增加,且第1周释放量最多。结论:牙龈卟啉单胞菌能在钛表面定植形成致密生物膜,引发钛表面氧化膜破坏和钛离子释放,该效应随细菌作用时间延长而加剧。

    Abstract:

    Objective:To study the effects of Porphyromonas gingivalis colonization on titanium surface oxide film properties. Methods:The samples of pure titanium were immersed in Porphyromonas gingivalis bacterial suspensions. Biofilm microstructure on the titanium surface was observed using scanning electron microscopy(SEM). The experimental groups were immersed in bacterial suspensions while the control group was immersed in physiological saline. After being co-cultured for 7,14,and 21 days,both samples and solutions were collected to analysis titanium surface properties using X-ray photoelectron spectroscopy(XPS) and to detect the quantity of titanium ion release using inductively coupled plasma optical emission spectrometer(ICP-OES),respectively. Results:SEM observed a dense biofilm after Porphyromonas gingivalis colonized on the titanium surface. With co-culture time increased,the XPS survey spectra showed reductions in the surface levels of titanium and oxygen,corresponding with an evident reduction of dominant titanium dioxide (TiO2) on the titanium surface revealed by the XPS high resolution spectra. ICP-OES indicated a increase of titanium ion release after colonization of Porphyromonas gingivalis on the titanium surface with the time extension,and the fastest release rate happened in the first week. Conclusion:Porphyromonas gingivalis was able to colonize on the titanium surface and form a dense biofilm. Porphyromonas gingivalis colonization weakened the protective TiO2 film on the titanium surface and induced the release of titanium ions,which enhanced with the bacterial action time prolonged.

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明盼盼,邵水易,邱 憬.牙龈卟啉单胞菌定植对钛表面氧化膜特性的影响[J].南京医科大学学报(自然科学版),2016,(10):1249-1253

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  • 收稿日期:2016-04-29
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  • 在线发布日期: 2016-10-17
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