文章摘要
赵向阳,石永强,缪 林,陈 跃.重组东亚钳蝎镇痛抗肿瘤肽协同5-氟尿嘧啶对小鼠H22肝癌的抑制作用[J].南京医科大学学报,2017,(1):44~47
重组东亚钳蝎镇痛抗肿瘤肽协同5-氟尿嘧啶对小鼠H22肝癌的抑制作用
Inhibition of recombinant analgesic-antitumor peptide combined with 5-flurouracil on H22 hepatoma in mice
投稿时间:2016-08-03  
DOI:10.7655/NYDXBNS20170109
中文关键词: 重组东亚钳蝎镇痛抗肿瘤肽  5-氟尿嘧啶  肝癌H22
英文关键词: recombinant analgesic-antitumor peptide  5-fluorouracil  H22 hepatoma
基金项目:南京市卫生局自然科学课题(YKK13200)
作者单位
赵向阳 南京市溧水区人民医院消化科江苏 溧水 211200 
石永强 南京市溧水区人民医院消化科江苏 溧水 211200 
缪 林 南京医科大学第二附属医院消化医学中心江苏 南京 210011 
陈 跃 南京市溧水区人民医院消化科江苏 溧水 211200 
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中文摘要:
      目的:探讨重组东亚钳蝎镇痛抗肿瘤肽(recombinant analgesic-antitumor peptide,rAGAP)增强5-氟尿嘧啶(5-fluorouracil,5-FU)对肝癌H22细胞的抑制作用及机制。方法:SPF级雄性小鼠120只,采用接种H22肝癌小鼠腹水方法建立模型,随机分为4组:rAGAP组(予rAGAP 0.03 mg/kg)、5-FU组(予5-FU 10 mg/kg)、联合组(予rAGAP 0.03 mg/kg, 5-FU 10 mg/kg),模型组(予等体积生理盐水),每4 d腹腔注射1次,用药持续24 d。用药结束后取出肿瘤组织标本,检测各组瘤体组织重量和抑瘤率,Western blot方法检测PI3K、AKT、PTEN蛋白表达。结果:与模型组相比,其他各组小鼠瘤体组织重量均明显降低(P<0.05);联合组抑瘤率明显高于rAGAP组和5-FU组(P<0.05)。与模型组相比,其他各组PI3K、p-Akt表达均明显减弱,PTEN表达明显增强(P<0.05);与5-FU组、rAGAP组相比,联合组PI3K、p-Akt表达均明显减弱,PTEN表达明显增强(P<0.05)。结论:rAGAP能增强5-FU对肝癌组织的抑制作用,其机制可能与影响PI3K/AKT/PTEN信号通路,进而抑制肝癌细胞的增殖有关。
英文摘要:
      Objective: To investigate the inhibitory effect and mechanism of recombinant analgesic-antitumor peptide (rAGAP) enhanced 5-fluorouracil(5-FU) on H22 hepatoma. Methods: The models of H22 hepatoma in mice were established through ascites inoculating H22 cells suspension into mice in right armpits, and randomly divided into 4 groups. rAGAP group was given 0.03 mg/kg IP rAGAP, 5-FU group 10 mg/kg IP 5-FU, United group 0.03 mg/kg 5-FU and 10 mg/kg IP rAGAP, Model group used equal volume of normal saline intraperitoneal injection for 3 weeks. After that, the tumor tissue were sampled, the tumor weight and tumor inhibition rate were detected. The expressions of PI3K, AKT, and PTEN were detected by Western blot method. Results: Compared with Model group, mice tumor weight of other groups was significantly lower (P<0.05); tumor suppressor rate of United Group was obviously higher than that of rAGAP group and 5-FU group (P<0.05). Compared with Model group, PI3K and p-Akt expression of other groups were significantly decreased, but PTEN expression was significantly increased (P<0.05); compared with 5-FU group, rAGAP group, PI3K and p-Akt expression of United group were significantly reduced, but PTEN expression was significantly increased (P<0.05). Conclusion: rAGAP can enhance the inhibitory effect of 5-FU on H22 hepatoma, its mechanism may be related to the activity of PI3K/AKT/PTEN signaling pathway, and then inhibition the proliferation of H22 hepatoma.
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