文章摘要
蔡建武,陈 辉,蔡宁宇,李 斌,潘 骏.p27在调节骨量和骨转换中的作用及机制研究[J].南京医科大学学报,2017,(1):53~57
p27在调节骨量和骨转换中的作用及机制研究
p27 plays an important role in modulating bone mass and bone turnover
投稿时间:2016-03-25  
DOI:10.7655/NYDXBNS20170111
中文关键词: p27  骨髓抽除  骨量  骨转换
英文关键词: p27  bone marrow ablation  bone mass  bone turnover
基金项目:温州市“551人才工程”项目
作者单位
蔡建武 温州医科大学附属第三医院外科浙江 温州 325000 
陈 辉 温州医科大学附属第二医院骨科浙江 温州 325000 
蔡宁宇 温州医科大学附属第二医院骨科浙江 温州 325000 
李 斌 温州医科大学附属第二医院骨科浙江 温州 325000 
潘 骏 温州医科大学附属第二医院骨科浙江 温州 325000 
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中文摘要:
      目的:明确p27在调节骨量和骨转换中的作用及机制。方法:选择同窝8周龄p27基因敲除(p27-/-)和同窝野生型(WT)小鼠胫骨和股骨行骨髓抽除术,利用影像学和组织病理学方法比较分析了术后3、7、11、14 d上述两组小鼠骨发生和骨转换的差异。结果:骨髓抽除术后3 d,WT和p27-/-小鼠骨髓均无明显新骨形成,但术后7、11、14 d,p27-/-小鼠骨髓中的新形成骨量均较WT小鼠明显增加。进一步研究发现,与WT小鼠相比,术后7、11、14 d,p27-/-小鼠骨髓腔中成骨细胞数和Ⅰ型胶原阳性面积均明显增加,而破骨细胞数和破骨细胞面均明显减少。结论: p27能够通过抑制成骨细胞骨形成,促进破骨细胞骨吸收,从而减少骨量并抑制骨转换。
英文摘要:
      Objective: To determine whether p27 plays an important role in modulating bone mass and bone turnover. Methods: We used 8-weeks-old p27 homozygote (p27-/-) mice and WT littermate mice. Firstly, we performed bone marrow ablation (BMX) on both tibia and femur, and then we compared the difference between osteogenesis and bone turnover of the different groups by radiography and pathologic histology. Finally, we detected the difference of ostosis and bone turnover between groups on the 3rd/7th/11th/14th day after BMX respectively. Results: Three days after BMX, the bone mass of both WT and p27-/- littermate showed no new osteogenesis. While compared to WT littermate, on the 7th/11th/14th day after BMX, the new osteogenesis in p27-/- mice increased significantly. Further study showed that the number of osteoblasts and the positive area of type I collagen increased significantly. However, the number of osteoclasts and the positive area of osteoclasts decreased markedly. Conclusion: The BMX of p27 gene knockout mice study proved that p27 could reduce the bone mass and inhibit bone turnover by suppressing the osteogenesis of osteoblasts and promoting the bone resorption of osteoclasts.
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