文章摘要
王武琴,吴 梦,陈 献,娄鉴芳,黄珮珺,黄 蕾,孙瑞红,王 芳.卵巢癌患者外周血CD4+调节T细胞与TGF-β1水平的相关性研究[J].南京医科大学学报,2017,(1):69~72
卵巢癌患者外周血CD4+调节T细胞与TGF-β1水平的相关性研究
Correlation between CD4+ regulatory T cells and TGF-β1 in peripheral blood of patients with epithelial ovarian cancer
投稿时间:2016-04-18  
DOI:10.7655/NYDXBNS20170114
中文关键词: 卵巢癌  CD4+调节T细胞  TGF-β1
英文关键词: ovarian cancer  CD4+ regulatory T cells  TGF-β1
基金项目:国家自然科学基金(81272324,81371894);江苏省实验诊断学重点实验室基金 (XK201114)
作者单位
王武琴 南京市江宁区中医院检验科江苏 南京 211100 
吴 梦 南京医科大学第一附属医院检验学部江苏 南京 210029 
陈 献 南京医科大学第一附属医院检验学部江苏 南京 210029 
娄鉴芳 南京医科大学第一附属医院检验学部江苏 南京 210029 
黄珮珺 南京医科大学第一附属医院检验学部江苏 南京 210029 
黄 蕾 南京医科大学第一附属医院检验学部江苏 南京 210029 
孙瑞红 南京医科大学第一附属医院检验学部江苏 南京 210029 
王 芳 南京医科大学第一附属医院检验学部江苏 南京 210029 
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中文摘要:
      目的:探讨卵巢癌患者外周血中CD4+CD25+及CD4+CD25+Foxp3+调节性T细胞的水平及其与转化生长因子β1(transforming growth factor-β1, TGF-β1)水平的相关性。方法:采用流式细胞术对24例上皮性卵巢癌患者外周血中CD4+CD25+及CD4+CD25+Foxp3+调节性T细胞水平进行检测,收集每例患者对应的血浆,ELISA法检测卵巢癌患者血浆中TGF-β1浓度,并选20例卵巢良性疾病及20例健康体检者做对比研究。结果:与卵巢良性疾病及健康对照组相比,卵巢癌患者CD4+CD25+、CD4+CD25+Foxp3+调节性T细胞及TGF-β1的表达水平明显增高(均P<0.01);Ⅲ~Ⅳ期卵巢癌患者CD4+CD25+、CD4+CD25+Foxp3+调节性T细胞及TGF-β1的表达水平明显高于Ⅰ~Ⅱ期患者(P<0.05);Pearson相关性分析结果显示,卵巢癌患者外周血CD4+CD25+及CD4+CD25+Foxp3+调节性T细胞水平与血浆TGF-β1水平均呈正相关(r=0.656, P<0.05; r=0.574, P<0.05)。结论:卵巢癌患者外周血存在高水平CD4+调节T细胞,且其与血浆TGF-β1水平呈正相关。检测外周血CD4+调节T细胞与TGF-β1水平对于判断卵巢癌不同病期有重要意义。TGF-β1水平增高可能参与调节CD4+调节T细胞对卵巢癌机体的免疫负调节机制。
英文摘要:
      Objective: To investigate the correlation between CD4+CD25+ and CD4+CD25+Foxp3+ regulatory T cells (Treg) and transforming growth factor-β1 (TGF-β1) in peripheral blood of patients with epithelial ovarian cancer. Methods: The percentages of CD4+CD25+ and CD4+CD25+Foxp3+ Treg in peripheral blood from 24 patients with ovarian cancer were detected by flow cytometry. Plasma level of TGF-β1 in each patient was detected by enzyme-linked immunosorbent assay (ELISA), and compared with that in 20 patients with benign ovarian tumor and 20 healthy volunteers. Results: The percentages of CD4+CD25+, CD4+CD25+Foxp3+ Treg cells and TGF-β1 in ovarian cancer patients were significantly higher than those in 20 patients with benign ovarian tumor and 20 healthy volunteers (both P<0.01). Levels of CD4+CD25+, CD4+CD25+Foxp3+ Treg cells and TGF-β1 in ovarian cancer patients at stage Ⅲ~Ⅳ were significantly higher than those of patients at stage Ⅰ~Ⅱ(P<0.05). Pearson correlation analysis showed that the percentages of CD4+CD25+ and CD4+CD25+Foxp3+ Treg cells were positively correlated with the levels of TGF-β1. Conclusion: The percentages of CD4+ Treg cells in peripheral blood from ovarian cancer were higher and positively correlated with TGF-β1 levels. Detection of CD4+ Treg cells and TGF-β1 has the clinical value on evaluation of development and prognosis of different stages of ovarian cancer. The increased levels of TGF-β1 may be involved in the immune negative adjustment mechanism of CD4+ Treg cells.
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