Objective:To develop a simple,low cost,portable and easy to realize automation pathogen nucleic acid detection system to meet the rapid on-site testing requirements. Methods:An enclosed cartridge was designed for integrated pathogen nucleic acid testing and creatively manufactured using 3D printing technology that speeded up the development cycle and saved the cost of the whole system. On this basis,a fully automatic portable system was developed,which now is able to automatically and integratedly realize single or multiple nucleic acid detection. Results:Various tests of this instrument were carried out,induding airtightness of cartridge and pipetting performance,blending performance,etc. All test results met the requirements of pathogen detection. At last,take pertussiss for example,the practical applications of nucleic acid detection showed that the cartridge system can complete nucleic acid testing automatically. Conclusion:This system can successfully and automatically complete the integration testing process of infectious diseases from sample input to result output. The experimental results are accurate and reliable.