文章摘要
吴 昊,陈俊杰,蒋 雷,孙 昊,王 军,张劲松.心脉隆对肺动脉平滑肌细胞缺氧损伤的保护作用[J].南京医科大学学报,2017,(2):174~178
心脉隆对肺动脉平滑肌细胞缺氧损伤的保护作用
Effect of XinMaiLong on human pulmonary smooth muscle cells exposed to hypoxia
投稿时间:2016-03-23  
DOI:10.7655/NYDXBNS20170208
中文关键词: 心脉隆  肺动脉平滑肌细胞  缺氧
英文关键词: Xinmailong injection(XML)  pulmonary artery smooth muscle cell  hypoxia
基金项目:国家自然科学基金(81273115,81473012);江苏省高校优势学科建设工程资助项目;江苏省中医局项目(LZ11081)
作者单位
吴 昊 南京医科大学第一附属医院急诊科,江苏 南京 210029 
陈俊杰 宜兴市人民医院ICU,江苏 宜兴 214200 
蒋 雷 南京医科大学第一附属医院急诊科,江苏 南京 210029 
孙 昊 南京医科大学第一附属医院急诊科,江苏 南京 210029 
王 军 南京医科大学公共卫生学院卫生部毒理实验室,江苏 南京 210029 
张劲松 南京医科大学第一附属医院急诊科,江苏 南京 210029 
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中文摘要:
      目的:研究心脉隆(XML)注射液对人源性肺动脉平滑肌细胞(PASMCs)缺氧损伤的保护作用,探讨XML降低肺动脉高压(PAH)的可能机制。方法:利用Na2S2O4建立缺氧模拟肺动脉高压细胞缺氧模型,流式细胞术观察缺氧刺激下细胞凋亡率,实时定量PCR检测凋亡相关基因表达,采用激光共聚焦法检测细胞膜电位?胞浆钙离子的表达变化。结果:在缺氧情况下,与对照组比较,PASMCs 增殖显著,Bcl-2基因表达增高,Bax表达下调,具有显著性差异(P < 0.05)。而XML能显著抑制缺氧过程中Bcl-2基因表达上调及Bax基因表达的下调,差异具有统计学意义(P < 0.05)。此外,XML能稳定细胞膜电位和降低胞浆钙浓度,抑制缺氧诱导的PASMCs增殖。结论:XML可减轻因缺氧引发的PASMCs凋亡减少而导致的平滑肌增殖重塑,为XML用于临床肺动脉高压的治疗提供实验依据。
英文摘要:
      Objective:To investigate the response of human pulmonary artery smooth muscle cells(PASMCs)to hypoxia with or without Xinmailong injection(XML),and explore the mechanism for XML to remit pulmonary artery hypertension(PAH). Methods:Na2S2O4 was used for building the hypoxic pulmonary artery smooth muscle cell model. The changes of apoptosis rate and apoptosis associated genes were detected by flow cytometry and realtime PCR,respectively,and membrane potentials and cytoplasmic Ca2+ under hypoxic stimulation were investigated by a laser scanning confocal microscope. Results:Under hypoxia condition,the proliferation of PASMCs was significant compared with that of the control group(P < 0.05),however,this effects can be markedly retarded by the treatment of XML(P < 0.05). Moreover,under hypoxia condition,Bcl-2 expression in PASMCs was obviously increased,while Bax expression was decreased when compared with those of the control group(P < 0.05). Noteworthily,XML can reverse both Bcl-2 and Bax expression induced by hypoxia(P < 0.05). In addition,XML stabilized the membrane potential and reduced the cytoplasmic Ca2+ under the hypoxic condition. Conclusion:Hypoxia leads to the proliferation of PASMCs,depolarization of membrane and increase of cytoplasmic Ca2+. The Chinese medicine XML can reduce all the effects induced by hypoxia,thereby our work might provide the experimental evidence for therapeutic strategy for pulmonary artery hypertension.
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