文章摘要
杨玉琳,陶文剑,唐宗湘弘,艾炳蔚.针刺通过降低TNF-α表达缓解脂多糖诱导的炎性痛[J].南京医科大学学报,2017,(3):303~306
针刺通过降低TNF-α表达缓解脂多糖诱导的炎性痛
Acupuncture relieves lipopolysaccharide (LPS) induced inflammatory pain by deregulating of TNF-α
投稿时间:2016-07-28  
DOI:10.7655/NYDXBNS20170309
中文关键词: 针刺  LPS  慢性炎症  TRPV1  TNF-α
英文关键词: acupuncture  LPS  chronic inflammation  TRPV1  TNF-α
基金项目:国家自然科学基金(31271181);江苏省高校“青蓝工程”优秀科技创新团队项目;江苏省中西医结合优势学科项目:南京中医药大学江苏省中医药防治肿瘤协同创新中心项目
作者单位
杨玉琳 南京中医药大学第二临床医学院江苏南京210023 
陶文剑 南京中医药大学基础医学院江苏南京210046南京医科大学第一附属医院针灸科江苏 南京210029 
唐宗湘弘 南京中医药大学基础医学院江苏南京210046 
艾炳蔚 南京中医药大学附属医院针灸康复科江苏 南京210029 
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中文摘要:
      目的:探讨针刺对脂多糖(lipopolysaccharide,LPS)诱导的慢性炎症疼痛行为的影响以及对慢性炎症的改善机制。方法:将18只6-8周龄C57BL/6J雄性小鼠随机分为3组:对照组小鼠腹腔注射4 mL/kg生理盐水;模型组小鼠腹腔注射LPS 3 m~kg,制造慢性炎症小鼠模型;针刺组慢性炎症小鼠给予针刺双侧“足三里”穴。3组小鼠不同处理后,分别采用Von Frev检测各组小鼠的机械缩足反射闽值(mechanical withdrawal threshold,MWT),采用酶联免疫法检测各组小鼠肿瘤坏死因子α(tumor necrosis f'actor,TNF-α)含量,采用杂交Westem blot检测各组小鼠背根神经节(dorsal root ganglion,DRCJ)神经无与热痛相关香草酸瞬时受体亚型1蛋白(transient receptor potential vanilloid 1,TRPV1)表达的差异。结果:针刺组与模型组相比较,小鼠机械痛闽明显升高(P<0.05),血清TNF-α含量明显降低(P<0.05),DRG神经元TRPV1蛋白表达降低(P<0.05)。结论:针刺治疗可以减少机体炎症因子的表达,同时也改变DRG神经元中TRPV1的表达,从而缓解和抑制慢性炎症所导致的疼痛。
英文摘要:
      Objective:To study the effects of acupuncture on pain behavior and the mechanism of acupuncture on improving chronic inflammation. Methods: We randomly divided 18 C57BL/6J male mice aged 6 to 8 weeks into three groups. The model and the acupuncture groups were administered with 3 mg/kg lipopolysaccharide (LPS) by intraperitoneal injection to induce chronic inflammation.while the control was administered with 4 ml/kg saline by intraperitoneal injection. The acupuncture group was treated by acupuncture at bilateral zusanli points (ST 36). After different treatments,Von Frey fiber filament was performed to detect mechanical withdrawal threshold(MWT) of the mice in each group. The content of tumor necrosis factor-α(TNF-α) in each group was detected by euzymelinked immunosorbent assay (ELISA). Bilateral dorsal root ganglion (DRG) neurons were performed to detect the heat pain related transient receptor potential vanilloid 1 (TRPV1) expression differences by Westem blot. Results: Compared with the model group, acupuncture increased the MWT significantly (P<0.05) .decreased the serum TNF-α content significantly (P<0.05) and TRPV1 protein expression of DRG neurons(P<0.05). Conclusion:Acupuncture treatment could reduce the expression of inflammatory cytokines, but also changed the expression of TRPV1 in DRG neurons,so as to suppress the pain caused by chronic inflammation.
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