文章摘要
王琛琛,金 晖,施毕旻,孙子林.二甲双胍对人血清诱导的胰腺癌细胞增殖及MMPs表达的影响[J].南京医科大学学报,2017,(4):419~422
二甲双胍对人血清诱导的胰腺癌细胞增殖及MMPs表达的影响
Effects of metformin on proliferation and expression of MMPs of cancer cells treated by human diabetic serum
投稿时间:2016-06-12  
DOI:10.7655/NYDXBNS20170407
中文关键词: 二甲双胍  糖尿病  胰腺肿瘤  增殖
英文关键词: metformin  diabetes mellitus  pancreatic neoplasms  proliferation
基金项目:国家高技术发展计划(863计划)(2015AA020314)
作者单位
王琛琛 东南大学附属中大医院内分泌科江苏 南京 210009 
金 晖 东南大学附属中大医院内分泌科江苏 南京 210009 
施毕旻 苏州大学附属第一医院内分泌科江苏 苏州 215006 
孙子林 东南大学附属中大医院内分泌科江苏 南京 210009 
摘要点击次数: 439
全文下载次数: 436
中文摘要:
      目的:探讨二甲双胍对糖尿病患者血清诱导的胰腺癌细胞株Patu8988增殖的影响及相关基因表达的变化。方法:以糖尿病患者血清诱导细胞株Patu8988为糖尿病组,以健康人血清为对照组,用不同浓度的二甲双胍(2.5、5.0、10.0、20.0、40.0 mmol/L)干预两组细胞,通过CCK-8细胞增殖实验检测不同血清诱导后细胞的增殖能力,采用RT-PCR检测二甲双胍干预后基质金属蛋白酶(MMP)-2、MMP-8和MMP-11 mRNA表达的差异。结果:与健康对照组相比,糖尿病患者血清诱导后促进Patu8988细胞增殖(0.74±0.03 vs.0.62±0.02,P<0.01);二甲双胍抑制糖尿病患者血清诱导的细胞增殖(P<0.01);RT-PCR结果显示二甲双胍减弱MMP-2、MMP-8、MMP-11的mRNA表达。结论:二甲双胍抑制糖尿病患者血清诱导的细胞增殖,这种抑制效应可能与其对MMP-2、MMP-8、MMP-11mRNA表达的下调作用有关。
英文摘要:
      Objective:To observe effects of metformin on proliferation and related gene expression of human pancreatic cancer cell line Patu8988 treated by diabetic serum.Methods:Patu8988 cells were cultured with diabetic serum,or healthy human serum as control.The two groups were treated with different concentrations of metformin (2.5,5.0,10.0,20.0,and 40.0 mmol/L.The proliferation was measured by cell counting kit-8 (CCK-8) assay,and the expressions of matrix metalloproteinase-2 (MMP-2),matrix metalloproteinase-8 (MMP-8) and matrix metalloproteinase-11 (MMP-11) were examined by real-time PCR.Results:Compared with the control group,the proliferation of Patu8988 was promoted after treatment with diabetic serum (0.74±0.03 vs.0.62±0.02,P<0.01):Metformin inhibited the proliferation of Patu8988 treated with diabetic serum (P<0.01),and the expressions of MMP-2,MMP-8 and MMP-11 were downregulated.Conclusion:Metformin inhibits the proliferation of cancer cells induced by the serum from patients with diabetes,which may be related to the down regulation of MMP-2,MMP-8, and MMP-11.
查看全文   查看/发表评论  下载PDF阅读器
关闭