文章摘要
蔡 运,陈 浩,张欣欣,李 皓.ABCA6缺失对生酮饮食喂养的小鼠代谢的影响[J].南京医科大学学报,2017,(7):804~808
ABCA6缺失对生酮饮食喂养的小鼠代谢的影响
Deletion of ATP binding cassette transporter A6 affects the metabolic status of mice fed on a ketogenic diet
投稿时间:2017-03-15  
DOI:10.7655/NYDXBNS20170704
中文关键词: ABCA6  生酮饮食  脂质代谢
英文关键词: ABCA6  ketogenic diet  lipid metabolism
基金项目:国家自然科学基金(81270944)
作者单位
蔡 运 南京医科大学病理生理学系江苏 南京 211166 
陈 浩 南京医科大学病理生理学系江苏 南京 211166 
张欣欣 南京医科大学病理生理学系江苏 南京 211166 
李 皓 南京医科大学病理生理学系江苏 南京 211166 
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中文摘要:
      目的:检测ATP转运蛋白6(ATP binding cassette transporter A6,ABCA6)基因缺失对生酮饮食喂养的小鼠代谢的影响。方法:给予小鼠生酮饮食(ketogenic diet,KD)或普通饮食(normal diet,ND)2周,通过Western blot检测肝脏ABCA6的表达水平;给予ABCA6敲除(KO)小鼠或对照(WT)小鼠KD 或ND 2周,检测小鼠的体重、血糖、血清甘油三酯(TG)、胆固醇(TCH)、游离脂肪酸(FFA)、酮体(β-HB)和转氨酶(AST、ALT)等指标,测定肝脏TG和TCH含量,并通过real-time PCR 检测代谢相关基因的表达水平。结果:生酮饮食诱导小鼠肝脏ABCA6的表达;在普通饮食条件下,ABCA6 KO小鼠与对照小鼠在体重、血糖及血清TG、TCH、FFA、β-HB等代谢相关指标、肝脂质含量和代谢相关基因表达水平等方面无明显差别;在生酮饮食条件下,和WT小鼠相比,ABCA6 KO小鼠的血糖、FFA和TCH降低,肝脏TG蓄积更显著,肝脏FAS、DGAT2、PCK1和HMGCS2 mRNA显著增加。结论:ABCA6缺失引起小鼠对生酮饮食的代谢障碍,ABCA6可能参与肝脏的脂质代谢过程。
英文摘要:
      Objective:To investigate the effect of ATP binding cassette transporter A6 (ABCA6) deletion on the metabolic status of mice fed on a ketogenic diet. Methods:ABCA6 knockout (KO) or wild type (WT) mice were placed on normal diet or ketogenic diet for 2 weeks,respectively. Expression of ABCA6 in the liver of mice fed on normal diet or ketogenic diet was determined by Western blot. Body weights were monitored. The blood samples and livers were harvested. Blood glucose and serum triglycerides (TG),total cholesterol (TCH),free fatty acids (FFA),ALT,AST,and beta hydroxybutyric acid (β-HB) were tested. The contents of TG and TCH in the liver were examined. The hepatic expression levels of genes related to metabolism were detected by realtime-PCR. Results:Feeding of ketogenic diet induced expression of ABCA6 in the liver. No significant difference was observed in body weight,blood glucose and the levels of TG,TCH,FFA,ALT,AST,and β-HB in serum of ABCA6 KO or WT mice fed on normal diet. A 2-week ketogenic diet feeding significantly reduced the levels of blood glucose,serum TCH,and serum FFA in ABCA6 KO mice when compared with WT mice. Meanwhile,more TG and higher mRNA levels of FAS,DGAT2,PCK1,and HMGCS2 were detected in the liver of ABCA6 KO mice fed on ketogenic diet. Conclusion:Deletion of ABCA6 impaired the metabolism of mice fed on ketogenic diet. ABCA6 may be involved in the lipid metabolism of the liver.
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