文章摘要
张鸿毅,高继学,汪 峰,李 义,贺晓龙,张培波,崔 洁.LRPPRC对前列腺癌细胞凋亡的影响[J].南京医科大学学报,2017,(7):847~851
LRPPRC对前列腺癌细胞凋亡的影响
Effects of LRPPRC on apoptosis in prostate cancer cells
投稿时间:2016-10-25  
DOI:10.7655/NYDXBNS20170712
中文关键词: 前列腺癌  LRPPRC  Bcl-2  凋亡
英文关键词: prostate cancer  LRPPRC  Bcl-2  apoptosis
基金项目:延安市科学技术研究发展计划项目(2015KW-06)
作者单位
张鸿毅 西安医学院第一附属医院泌尿外科陕西 西安 710077延安大学附属医院泌尿外科陕西 延安 716000 
高继学 延安大学附属医院泌尿外科陕西 延安 716000 
汪 峰 延安大学附属医院泌尿外科陕西 延安 716000 
李 义 延安大学附属医院泌尿外科陕西 延安 716000 
贺晓龙 延安大学附属医院泌尿外科陕西 延安 716000 
张培波 延安大学附属医院泌尿外科陕西 延安 716000 
崔 洁 西安医学院第一附属医院肿瘤科陕西 西安 710077 
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中文摘要:
      目的:分析富含亮氨酸的三角状五肽重复结构蛋白(LRPPRC)和抗凋亡蛋白Bcl-2在前列腺癌组织的表达及LRPPRC对前列腺癌细胞凋亡的影响。方法:应用免疫组化法检测198例前列腺癌组织中LRPPRC和Bcl-2的表达;分析LRPPRC和Bcl-2表达的相关性;分析LRPPRC和Bcl-2表达与预后的关系;应用siRNA LRPPRC转染前列腺癌细胞下调LRPPRC水平,观察对细胞增殖、凋亡和Bcl-2、Bax、caspase-3蛋白表达的影响。结果:前列腺癌和前列腺增生组织LRPPRC的表达率分别为61.6%(122/198)和18.0%(18/100)(P<0.001);Bcl-2的表达率分别为49.5%(98/198)和45.0%(45/100)(P=0.539)。LRPPRC和Bcl-2的表达有显著正相关性(r=0.179,P=0.012)。LRPPRC和Bcl-2同时高表达的患者无疾病进展生存和总生存期均显著降低(P<0.001)。下调LRPPRC表达可抑制前列腺癌细胞增殖,促进前列腺癌细胞凋亡,降低Bcl-2蛋白的表达,使caspase-3活化增多,对Bax表达无显著影响。结论:前列腺癌组织LRPPRC表达显著增高;LRPPRC与抑制凋亡有关;LRPPRC在前列腺癌中的作用机制与抗凋亡蛋白Bcl-2有关。
英文摘要:
      Objective:To investigate the expressions of LRPPRC and Bcl-2 in prostate cancer,and the effects of LRPPRC on apoptosis. Methods:Immunohistochemistry was performed to analyze the expressions of LRPPRC and Bcl-2 on 198 prostate cancer tissues. The correlation between LRPPRC and Bcl-2 expressions was analyzed. The relationship between LRPPRC and Bcl-2 expressions and prognosis was analyzed. siRNA LRPPRC was used to transfect prostate cancer cells to decrease the level of LRPPRC,and the effects on proliferation,apoptosis,the expressions of Bcl-2,Bax and caspase-3 protein were observed. Results:Prostate cancer and prostate hyperplasia tissues highly expressed LRPPRC in 61.6%(122/198) and 18.0%(18/100) of patients,respectively(P<0.001). Prostate cancer and prostate hyperplasia tissues highly expressed Bcl-2 in 49.5%(98/198) and 45.0%(45/100) of patients,respectively(P=0.539). A significant association between the expressions of LRPPRC and Bcl-2 was observed(r=0.179;P=0.012). High expression of LRPPRC combined with Bcl-2 was related with shorter biochemical progress(BCP)-free survival and overall survival(P<0.001). When LRPPRC level being down-regulated by siRNA,the survival rate of prostate cancer cells was decreased,the apoptosis rate was increased,Bcl-2 protein level was decreased,and cleaved caspase-3 protein level was increased. There were no significant effects on Bax expression. Conclusion:LRPPRC expression was increased in prostate cancer tissues. LRPPRC may inhibit endogenous apoptosis of prostate cancer cells. The effect may be related with regulating Bcl-2 expression.
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