Expression of PARP14 in PDAC and its biological function
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摘要:
目的：检测PARP14在胰腺癌中的表达及意义，并探讨其对胰腺癌细胞生物学功能的影响。方法：利用人类肿瘤相关的基因表达汇编（gene expression omnibus, GEO）和癌症基因组图谱（the cancer genome atlas, TCGA）中的胰腺癌基因表达谱数据，分析PARP14在胰腺癌及癌旁中的表达差异，并进一步通过TCGA数据库分析PARP14表达量与总体生存期和无病生存期的关系（Kaplan-Meier法和Log-rank检验）。在人胰腺癌细胞株BXPC-3和CFPAC-1中干扰PARP14后，分别通过CCK8增殖实验和集落形成实验检测其对胰腺癌细胞增殖能力的影响。结果：GEO数据显示PARP14在胰腺癌组织中的表达显著高于其在癌旁组织中的表达，差异具有统计学意义（P<0.001）。TCGA数据表明胰腺癌中高表达PARP14组患者的总体生存期显著低于低表达组的患者（P<0.05）。体外实验表明，干扰PARP14后，胰腺癌细胞增殖能力明显降低（P<0.05）。结论：PARP14在胰腺癌中高表达并与预后负相关，且其高表达与胰腺癌细胞增殖能力相关，PARP14可能成为胰腺癌预后预测及治疗的靶点。
Abstract:
Objective: To investigate the expression of PARP14 and its biological functions in pancreatic ductal adenocarcinoma (PDAC). Methods: The public gene chip data of PDAC obtained from the Gene Expression Omnibus (GEO) database and the Cancer Genome Atlas (TCGA) database were used to analyze the expression of PARP14 between pancreatic tumors and its adjacent tissues, The Kaplan-Meier method and Log rank test were used to assess survival analysis according to PARP14 expression in PDAC from TCGA. The biological role of PARP14 in regulating the proliferation of PDAC cells was evaluated by CCK-8 assay and colony formation experiment in BXPC-3 and CFPAC-1 cells transfected with PARP14 siRNAs. Results: The expression level of PARP14 in PDAC tissues were higher than that in the adjacent tissues or healthy controls according to the GEO databases (P<0.001). TCGA database further revealed that higher expression of PAPR14 was significantly associated with poorer survival of PDAC patients (P<0.05). Besides, PARP14 siRNAs were successfully transfected into PDAC cells BXPC-3 and CFPAC-1, and transfection with PARP14 siRNAs significantly suppressed proliferation of PDAC cells (P<0.05). Conclusion: The expression of PARP14 is up-regulated in PDAC tissues and high expression of PARP14 predicts poor survival, suggesting its role as a potential oncogene in PDAC.
