文章摘要
吴 靓,周旭婕,李小霞,石 靓,丁 强.RNPC1通过抑制Snail表达影响乳腺癌转移[J].南京医科大学学报,2017,(8):938~942
RNPC1通过抑制Snail表达影响乳腺癌转移
RNPC1 inhibits breast cancer metastasis via down-regulating Snail
投稿时间:2017-02-03  
DOI:10.7655/NYDXBNS20170804
中文关键词: RNPC1  乳腺肿瘤  淋巴结转移  Snail
英文关键词: RNPC1  breast cancer  lymph node metastasis  Snail
基金项目:国家自然科学基金目(81572595)
作者单位
吴 靓 南京医科大学第一附属医院乳腺外科江苏 南京 210029 
周旭婕 南京医科大学第一附属医院乳腺外科江苏 南京 210029 
李小霞 南京医科大学第一附属医院乳腺外科江苏 南京 210029 
石 靓 南京医科大学第一附属医院乳腺外科江苏 南京 210029 
丁 强 南京医科大学第一附属医院乳腺外科江苏 南京 210029 
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中文摘要:
      目的:研究人乳腺癌细胞BT474中RNPC1对Snail表达的调控,及其与乳腺癌淋巴结转移等生物学行为的关系。方法:采用免疫组化(SP法)检测42例伴有淋巴结转移及39例无淋巴结转移的乳腺癌组织中RNPC1蛋白的表达,并计算差值;通过TCGA数据库分析伴有或不伴有淋巴结转移的乳腺癌组织中RNPC1基因表达差异;采用慢病毒转染法干扰或过表达RNPC1基因,实时荧光定量PCR (qRT-PCR) 和Western blot法检测BT474细胞中Snail的表达量;以双荧光素酶报告基因实验证实RNPC1与靶基因Snail的结合。结果:与有淋巴结转移的乳腺癌组织相比,RNPC1的表达量在无淋巴结转移的组织中相对较高;干扰RNPC1能够明显增加BT474细胞中Snail的表达,过表达RNPC1可以显著降低Snail的表达;RNPC1通过结合Snail 3′-非编码区(3′-UTR)降低其表达水平。结论:RNPC1可以通过降低Snail的表达,影响乳腺癌的转移能力。
英文摘要:
      Objective: To explore the regulatory mechanism of RNPC1 on the expression of Snail family transcriptional repressor 1 (Snail) in BT474 breast cancer cells and the possible role of RNPC1 in breast cancer lymph node metastasis. Methods: Immunohistochemical (IHC) staining was used to detect and compare the expression of RNPC1 in 42 cases of breast cancer tissues with lymph node metastasis and 39 cases without lymphatic metastasis. The difference of RNPC1 gene level in breast cancer tissues with or without lymph node metastasis was calculated via utilizing TCGA database. The expression of RNPC1 in BT474 cells was knocked down or induced by lentiviral vector, and the effect was identified by qRT-PCR and Western blot. Dual-luciferase activity assay was applied to test and verify the target gene of RNPC1. Results: Compared with breast cancer tissues with lymph node metastasis, expression of RNPC1 was significantly higher in those without lymph node metastasis. The qRT-PCR and Western blot results showed that knockdown of RNPC1 in BT474 cells up-regulated Snail levels, whereas overexpression of RNPC1 resulted in down-regulation of Snail expressions. The dual-luciferase activity assay confirmed that 3′-untranslated region (3′-UTR) was required for RNPC1 to decrease the expression of Snail. Conclusion: Our findings suggested that RNPC1 might inhibit breast cancer metastasis via targeting Snail.
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