文章摘要
梁 婕,韩 莉,梁 健,凌 敏,卞 倩.let-7f-5p在不明原因复发性流产绒毛组织中的表达及其功能研究[J].南京医科大学学报,2017,(8):971~975
let-7f-5p在不明原因复发性流产绒毛组织中的表达及其功能研究
The mechanism study of enhancer RNA inducing inflammatory response in unexplained recurrent spontaneous abortion
投稿时间:2016-10-17  
DOI:10.7655/NYDXBNS20170810
中文关键词: 不明原因复发性流产  let-7f-5p  凋亡
英文关键词: URSA  let-7f-5p  apoptosis
基金项目:江苏省“333高层次人才培养工程”(BRA2015490)
作者单位
梁 婕 江苏省疾病预防控制中心毒理与功能评价所江苏 南京 210009 
韩 莉 南京医科大学附属淮安第一医院产科江苏 淮安 223300 
梁 健 南京医科大学附属淮安第一医院产科江苏 淮安 223300 
凌 敏 江苏省疾病预防控制中心毒理与功能评价所江苏 南京 210009 
卞 倩 江苏省疾病预防控制中心毒理与功能评价所江苏 南京 210009 
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中文摘要:
      目的:筛选与不明原因复发性流产(unexplained recurrent spontaneous abortion, URSA)相关的微小RNA(miRNA),探讨其在URSA发生中的作用及其可能机制。方法:通过对URSA绒毛组织进行miRNAs测序,初步筛选差异表达的miRNAs;利用实时荧光定量PCR,验证筛选所得miRNAs表达水平;利用DAVID软件对靶基因进行Pathway分析;利用实时荧光定量PCR,检测p53通路中靶基因及其下游基因的表达水平。结果:let-7f-5p在URSA绒毛组织中表达水平显著上升;let-7f-5p的靶基因富集于p53通路,通路中靶基因MDM-X和其下游靶标促生长基因MDM2水平显著下降,促凋亡基因p53表达水平显著上升。结论:let-7f-5p可能调控p53信号通路中MDM-X/MDM2/p53基因,促进细胞凋亡,最终导致URSA发生发展。
英文摘要:
      Objective: To screen the differently expressed miRNAs study the expression andfunction of let-7f-5p in unexplained recurrent spontaneous abortion (URSA) and investigate the roles of miRNAs played in URSA. Methods: The miRNA-sequencing was applied to screen miRNAs that differently expressed in URSA placental villous; The qRT-PCR was used to validate the miRNA’s expression of screened miRNAs; The DAVID was applied to analyze pathway; The qRT-PCR was conducted to measure the expression of target genes in p53 pathway. Results: The expression of let-7f-5p was markedly up-regulatedhighly expressed in URSA placental villous; The targets of let-7f-5p were abundant in p53 pathway, and the expression of MDM-X and MDM2 was significantly down-regulated, while the expression of p53 was significantly up-regulated. Conclusion: let-7f-5p might promote cell apoptosis in URSA by regulating the expression of MDM-X/MDM2/p53 in p53 pathway.
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